Multi-system approach to study mutagenesis induced by chemical carcinogens.

P. Campomenosi, R. Iannone, A. Inga, P. Menichini, P. Degan, A. Abbondandolo, G. Fronza

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To understand molecular mechanisms of the mutation fixation process induced by a mutagen and carcinogen, a multi-system approach is suggested to reduce the probability that the results are biased by the assay used. In this light we described our different approaches to answer basic questions on the mutagenesis induced by the chemical carcinogen 4-Nitroquinoline-1-oxide. We determined mutations at the molecular level in three experimental systems: a) in prokaryotes (ss M13mp19 lacZ'/E. coli F'lacZ delta M15); b) in eukaryotes (i) ss and ds pZ189 supF/CV1-P/E.coli lacZam and (ii) HPRT in CHO cells with different repair capacity. We think this type of approach can be used to study the genetic effects of new cancer drugs for which the molecular mechanisms of action at the molecular level are still not well understood. We think to apply the know-how to study mutational spectra in tumor derived tumor suppressor genes.

Original languageEnglish
Pages (from-to)699-705
Number of pages7
JournalBollettino della Societa Italiana di Biologia Sperimentale
Issue number11
Publication statusPublished - Nov 1992

ASJC Scopus subject areas

  • Medicine(all)


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