Multicenter comparison of ESP Culture System II with BACTEC 460TB and with Lowenstein-Jensen medium for recovery of mycobacteria from different clinical specimens, including blood

Enrico Tortoli, Paola Cichero, M. Gabriella Chirillo, M. Rita Gismondo, Letizia Bono, Giampietro Gesu, M. Tullia Simonetti, Gisella Volpe, Giampietro Nardi, Piero Marone

Research output: Contribution to journalArticlepeer-review

Abstract

The recently developed ESP Culture System II (AccuMed, Chicago, III.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tuberculosis complex, species. Rates of recovery obtained with BACTEC, ESP, and Lowenstein-Jensen medium were 89, 79, and 64%, respectively, with such differences being statistically significant. Different media and systems appeared to behave differently when the more frequently detected organisms were considered: M. tuberculosis complex isolates grew better with BACTEC, and MAC isolates grew better with ESP. An analysis of the combinations of Lowenstein-Jensen medium with BACTEC and with ESP did not reveal significant differences in recovery rates. With regard to the times needed for the detection of positive cultures, they were significantly longer on Lowenstein-Jensen medium (average, 28 days) than with the remaining two systems, between which there was no difference (average, 18 days). We conclude, therefore, that the ESP system, when used in combination with a solid medium, performs as well as the thoroughly validated radiometric BACTEC system and offers the advantages of full automation and absence of radioisotopes.

Original languageEnglish
Pages (from-to)1378-1381
Number of pages4
JournalJournal of Clinical Microbiology
Volume36
Issue number5
Publication statusPublished - May 1998

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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