TY - JOUR
T1 - Multicenter evaluation of analytical performances of platelet counts and platelet parameters
T2 - Carryover, precision, and stability
AU - Gioia, Maria
AU - Da Rin, Giorgio
AU - Manenti, Barbara
AU - Birindelli, Sarah
AU - Ciardelli, Maria Laura
AU - Gentile, Roberto
AU - Beretta, Gianluca
AU - Lorusso, Giuseppe
AU - Avino, Daniela
AU - Di Fabio, Anna Maria
AU - Dima, Francesco
AU - Fanelli, Alessandra
AU - Lorubbio, Maria
AU - Francione, Sara
AU - Marincheva, Galina
AU - Marini, Alessandra
AU - Papa, Angela
AU - Giannelli, Elena
AU - Pajola, Rachele
AU - Panzeri, Andrea
AU - Pipitone, Silvia
AU - Benegiamo, Anna
AU - Rolla, Roberta
AU - Vidali, Matteo
AU - Buoro, Sabrina
N1 - © 2020 John Wiley & Sons Ltd.
PY - 2020/10
Y1 - 2020/10
N2 - INTRODUCTION: The correctness of the results of automated platelet analysis is still highly debated. The aim of this multicenter study, conducted according to international guidelines, was to verify the analytical performance of nine different types of hematology analyzers (HAs) in the automated platelet analysis.METHODS: Four hundred eighty-six peripheral blood samples (PB), collected in K3 EDTA tubes, were analyzed by ABX Pentra, ADVIA2120i, BC-6800, BC-6800 Plus, Cell-DYN Sapphire, DxH800, XE-2100, XE-5000, XN-20 with PLT-F App. Within-run imprecision and between-run imprecision were carried out using PB and material control, respectively. The carryover, low limit of quantification (LoQ), and the PB stability were evaluated.RESULTS: The carryover was absent for all HAs. The LoQ of PLT ranged between 2.0 (Cell-Dyn Sapphire) and 25.0 × 109 /L (ADVIA 2120i), while immature platelet fraction (IPF) ranged between 1.0 (XN-20) and 12.0 × 109 /L (XE-5000). The imprecision (%CV) increases as the platelet count decreases. No HAs showed desirable CVAPS for PLT counts less than 50.0 × 109 /L, with the exception of Cell-DYN Sapphire (CV 3.0% with PLT-O mean value of 26.7 × 109 /L), XN-20 (CV 2.4% with PLT-F mean value of 21.5 × 109 /L), and BC-6800 Plus (CV 1.9% with PLT-O mean value of 26.5 × 109 /L). The sample stability ranged between under two hours for MPV by ADVIA2120i and 8 hours for other PLT parameters and HAs.CONCLUSION: The findings of this study may provide useful information regarding carryover, precision, and stability of platelet counts and parameters, especially in thrombocytopenic samples. Moreover, the stability of sample for platelet analysis is conditioned by the HA and by temperature and storage time.
AB - INTRODUCTION: The correctness of the results of automated platelet analysis is still highly debated. The aim of this multicenter study, conducted according to international guidelines, was to verify the analytical performance of nine different types of hematology analyzers (HAs) in the automated platelet analysis.METHODS: Four hundred eighty-six peripheral blood samples (PB), collected in K3 EDTA tubes, were analyzed by ABX Pentra, ADVIA2120i, BC-6800, BC-6800 Plus, Cell-DYN Sapphire, DxH800, XE-2100, XE-5000, XN-20 with PLT-F App. Within-run imprecision and between-run imprecision were carried out using PB and material control, respectively. The carryover, low limit of quantification (LoQ), and the PB stability were evaluated.RESULTS: The carryover was absent for all HAs. The LoQ of PLT ranged between 2.0 (Cell-Dyn Sapphire) and 25.0 × 109 /L (ADVIA 2120i), while immature platelet fraction (IPF) ranged between 1.0 (XN-20) and 12.0 × 109 /L (XE-5000). The imprecision (%CV) increases as the platelet count decreases. No HAs showed desirable CVAPS for PLT counts less than 50.0 × 109 /L, with the exception of Cell-DYN Sapphire (CV 3.0% with PLT-O mean value of 26.7 × 109 /L), XN-20 (CV 2.4% with PLT-F mean value of 21.5 × 109 /L), and BC-6800 Plus (CV 1.9% with PLT-O mean value of 26.5 × 109 /L). The sample stability ranged between under two hours for MPV by ADVIA2120i and 8 hours for other PLT parameters and HAs.CONCLUSION: The findings of this study may provide useful information regarding carryover, precision, and stability of platelet counts and parameters, especially in thrombocytopenic samples. Moreover, the stability of sample for platelet analysis is conditioned by the HA and by temperature and storage time.
KW - Blood Platelets/cytology
KW - Humans
KW - Italy
KW - Platelet Count/instrumentation
KW - Platelet Function Tests/instrumentation
KW - Reproducibility of Results
KW - Sensitivity and Specificity
U2 - 10.1111/ijlh.13204
DO - 10.1111/ijlh.13204
M3 - Article
C2 - 32304271
VL - 42
SP - 552
EP - 564
JO - Int. J. Lab. Hematol.
JF - Int. J. Lab. Hematol.
SN - 1751-5521
IS - 5
ER -