Valutazione multicentrica dei conteggi cellulari ottenuti con 8 analizzatori ematologici automatici

Translated title of the contribution: Multicenter evaluation of blood cell counts on 8 automated hematology analyzers

Sabrina Buoro, Silvia Pipitone, Alessandra Fanelli, Sara Francione, Giorgio Da Rin, Annamaria Di Fabio, Fabiana Fiorini, Alessandra Marini, Angela Papa, Michela Seghezzi, Anna Benegiamo, Benedetta Peruzzi, Marco Borin, Fosca Siviero, Lucia Francioni, Tiziana Lari, Franca Cocci, Fiamma Balboni, Maria Laura Ciardelli, Francesco DimaLuca Germagnoli, Maria Gioia, Antonio La Gioia

Research output: Contribution to journalArticlepeer-review


The cellular analysis performed on hematology analyzers is based on the interaction of cells with electrical or optical signals. The heterogeneity of adopted methods and technologies by different analyzers can translate in a lack of homogeneity in analytical performance. This study compares 8 hematological analyzers vs. optical microscopy (OM) and, where possible, also compares the analyzers among each other. Correlations were assessed by Pearson's coefficient of correlation, Passing and Bablock regression and Bland-Altman bias plot analysis. The comparison among analyzers regarding leukocyte differential counts showed a good level of agreement, except for the basophil cell count. For this "critical population", the bias ranged from -5,8% (Cell-Dyn Sapphire vs. XN-9000) to 30,6% (Advia 2120i vs. XE- 2100). The comparison between automated differential leukocyte counts and OM showed also a good level of agreement, with a bias ranging from -0,9% to 8.9%. The bias for basophil cell count was however very high (79.5%).

Translated title of the contributionMulticenter evaluation of blood cell counts on 8 automated hematology analyzers
Original languageItalian
Pages (from-to)195-203
Number of pages9
JournalBiochimica Clinica
Issue number3
Publication statusPublished - Jan 1 2016

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical


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