Multicenter evaluation of mycobacteria growth indicator tube (MGIT) compared with the BACTEC radiometric method, BBL biphasic growth medium and Lowenstein-Jensen medium

Enrico Tortoli, Ferruccio Mandler, Mirella Tronci, Valeria Penati, Giovanni Sbaraglia, Danila Costa, Giuseppe Montini, Massimo Predominato, Renato Riva, Cristiana Passerini Tosi, Claudio Piersimoni, Pasquale Urbano

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the new BBL mycobacteria growth indicator tube (MGIT) in comparison with other media. Methods: MGIT was evaluated in 10 Italian centers on 433 clinical samples, mainly of respiratory origin and mainly smear positive, in comparison with Lowenstein-Jensen and with one or more other methods represented, according to participating centers, by the BACTEC radiometric method or by the biphasic BBL Septi-Chek AFB system. While MGIT and Lowenstein-Jensen were used for all the samples, 285 of them were also inoculated in BACTEC vials and 274 in biphasic bottles. Of these samples, 132 were investigated with all the four methods. Results: Although less rapid and sensitive than the radiometric method, the results of MGIT were equal when compared with the other two media with respect to overall isolation yield; furthermore, it allowed the detection of growth in significantly shorter times. Conclusions: The results of this study indicate the value of MGIT for the detection of mycobacteria and, thanks to its extreme simplicity of use, its suitability for small and large laboratories. Its combined use with a solid medium can substantially improve the diagnosis of mycobacterial infection.

Original languageEnglish
Pages (from-to)468-473
Number of pages6
JournalClinical Microbiology and Infection
Volume3
Issue number4
Publication statusPublished - 1997

Keywords

  • Culture media
  • M. tuberculosis
  • Non-tuberculous mycobacteria

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Fingerprint Dive into the research topics of 'Multicenter evaluation of mycobacteria growth indicator tube (MGIT) compared with the BACTEC radiometric method, BBL biphasic growth medium and Lowenstein-Jensen medium'. Together they form a unique fingerprint.

Cite this