Multicenter validation of a reproducible flow cytometric score for the diagnosis of low-grade myelodysplastic syndromes: Results of a European LeukemiaNET study

Matteo G Della Porta, Cristina Picone, Cristiana Pascutto, Luca Malcovati, Hideto Tamura, Hiroshi Handa, Magdalena Czader, Sylvie Freeman, Paresh Vyas, Anna Porwit, Leonie Saft, Theresia M. Westers, Canan Alhan, Claudia Cali, Arjan A. van de Loosdrecht, Kiyoyuki Ogata

Research output: Contribution to journalArticle

Abstract

Background The current World Health Organization classification of myelodysplastic syndromes is based on morphological evaluation of bone marrow dysplasia. In clinical practice, the reproducibility of the recognition of dysplasia is usually poor especially in cases that lack specific markers such as ring sideroblasts and clonal cytogenetic abnormalities. Design and Methods We aimed to develop and validate a flow cytometric score for the diagnosis of myelodysplastic syndrome. Four reproducible parameters were analyzed: CD34+ myeloblast-related and B-progenitor-related cluster size (defined by CD45 expression and side scatter characteristics on CD34+ marrow cells), myeloblast CD45 expression and granulocyte side scatter value. The study comprised a "learning cohort" (n=538) to define the score and a "validation cohort" (n=259) to confirm its diagnostic value. Results With respect to non-clonal cytopenias, patients with myelodysplastic syndrome had increased myeloblast-related cluster size, decreased B-progenitor-related cluster size, aberrant CD45 expression and reduced granulocyte side scatter (P

Original languageEnglish
Pages (from-to)1209-1217
Number of pages9
JournalHaematologica
Volume97
Issue number8
DOIs
Publication statusPublished - Aug 1 2012

Keywords

  • Diagnosis
  • Flow cytometry
  • Myelodysplastic syndrome

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Multicenter validation of a reproducible flow cytometric score for the diagnosis of low-grade myelodysplastic syndromes: Results of a European LeukemiaNET study'. Together they form a unique fingerprint.

  • Cite this

    Porta, M. G. D., Picone, C., Pascutto, C., Malcovati, L., Tamura, H., Handa, H., Czader, M., Freeman, S., Vyas, P., Porwit, A., Saft, L., Westers, T. M., Alhan, C., Cali, C., van de Loosdrecht, A. A., & Ogata, K. (2012). Multicenter validation of a reproducible flow cytometric score for the diagnosis of low-grade myelodysplastic syndromes: Results of a European LeukemiaNET study. Haematologica, 97(8), 1209-1217. https://doi.org/10.3324/haematol.2011.048421