Multicentre standardisation of a clinical grade procedure for the preparation of allogeneic platelet concentrates from umbilical cord blood

Paolo Rebulla, Simonetta Pupella, Michele Santodirocco, Noemi Greppi, Ida Villanova, Marina Buzzi, Nicola De Fazio, Giuliano Grazzini, Marino Argiolas, Paola Bergamaschi, Maria Bianchi, Tiziana Bonfini, Daniela Bovo, Marina Buzzi, Mauro Carta, Pier Luigi Cocco, Lidia De Felice, Lazzaro Di Mauro, Giovanni Foti, Noemi GreppiMariacarla Iorio, Elisabetta Liberatore, Maurizio Marconi, Benedetta Mazzanti, Laura Mazzucco, Mario Pagano, Pasqualepaolo Pagliaro, Vincenzo Poggi, Giulia Pucci, Simonetta Pupella, Paolo Rebulla, Sara Rinalducci, Bina Romano, Riccardo Saccardi, Laura Salvaneschi, Michele Santodirocco, Valentina Schiavo, Maria Screnci, Serena Spartano, Angela Totaro, Patrizia Urciuoli, Stefania Vaglio, Claudio Velati, Ida Villanova, Lello Zolla

Research output: Contribution to journalArticlepeer-review


Background. In addition to a largely prevalent use for bleeding prophylaxis, platelet concentrates from adult blood have also been used for many years to prepare platelet gels for the repair of topical skin ulcers. Platelet gel can be obtained by activation of fresh, cryopreserved, autologous or allogeneic platelet concentrates with calcium gluconate, thrombin and/or batroxobin. The high content of tissue regenerative factors in cord blood platelets and the widespread availability of allogeneic cord blood units generously donated for haematopoietic transplant but unsuitable for this use solely because of low haematopoietic stem cell content prompted us to develop a national programme to standardise the production of allogeneic cryopreserved cord blood platelet concentrates (CBPC) suitable for later preparation of clinical-grade cord blood platelet gel. Materials and methods. Cord blood units collected at public banks with total nucleated cell counts 150×109/L and volume >50 mL, underwent soft centrifugation within 48 hours of collection. Platelet-rich plasma was centrifuged at high speed to obtain a CBPC with target platelet concentration of 800-1,200×109/L, which was cryopreserved, without cryoprotectant, below -40 °C. Results. During 14 months, 13 banks produced 1,080 CBPC with mean (± standard deviation) volume of 11.4±4.4 mL and platelet concentration of 1,003±229×109/L. Total platelet count per CBPC was 11.3±4.9×109. Platelet recovery from cord blood was 47.7±17.8%. About one-third of cord blood units donated for haematopoietic transplant could meet the requirements for preparation of CBPC. The cost of preparation was € 160.92/CBPC. About 2 hours were needed for one technician to prepare four CBPC. Discussion. This study yielded valuable scientific and operational information regarding the development of clinical trials using allogeneic CBPC.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalBlood Transfusion
Issue number1
Publication statusPublished - Jan 1 2016


  • Platelet concentrate
  • Platelet gel
  • Skin ulcers
  • Umbilical cord blood

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy


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