Multicentre study, in patients with imported malaria, on the sensitivity and specificity of a dipstick test (ICT Malaria P.f./P.v.™) compared with expert microscopy

S. Gatti, A. M. Bernuzzi, Z. Bisoffi, A. Raglio, M. Gulletta, M. Scaglia, F. Vailati, A. Matteelli, M. Moroni, R. Visonà, A. Orani, G. Perboni, A. Volonterio, L. Galimberti, G. Giani, G. Orlando, M. Anselmi, M. Gobbo, A. Maccabruni, A. MalfitanoC. Cevini, A. Bruno, R. Maserati, M. Giola

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A prospective, multicentre study was carried out in Italy to assess the sensitivity and specificity of a rapid dipstick test (ICT Malaria P.f./P.v.) in the diagnosis of imported malaria caused by Plasmodium falciparum and other Plasmodium spp. The test is based on the detection of histidine-rich protein-2 (HRP-2) from P. falciparum and 'panmalarial' antigen in peripheral blood. The 241 subjects were international travellers or immigrants from areas where malaria is endemic. When compared with the microscopical examination of bloodsmears (used as the 'gold standard'), the dipsticks were found to be 94.4% sensitive and 94.5% specific for pure infections with P. falciparum. The performance of the tests when used on patients infected with species other than P. falciparum or more than one Plasmodium spp. showed a high degree of variability. Although the dipsticks represent a very simple, rapid, and valuable diagnostic aid, they should not be considered a complete substitute for direct microscopical diagnosis using stained bloodsmears.

Original languageEnglish
Pages (from-to)15-18
Number of pages4
JournalAnnals of Tropical Medicine and Parasitology
Volume96
Issue number1
Publication statusPublished - 2002

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Plasmodium
Plasmodium falciparum
Malaria
Multicenter Studies
Microscopy
Sensitivity and Specificity
Falciparum Malaria
Italy
Prospective Studies
Antigens
Infection
Plasmodium falciparum HRP-2 antigen

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Immunology

Cite this

Multicentre study, in patients with imported malaria, on the sensitivity and specificity of a dipstick test (ICT Malaria P.f./P.v.™) compared with expert microscopy. / Gatti, S.; Bernuzzi, A. M.; Bisoffi, Z.; Raglio, A.; Gulletta, M.; Scaglia, M.; Vailati, F.; Matteelli, A.; Moroni, M.; Visonà, R.; Orani, A.; Perboni, G.; Volonterio, A.; Galimberti, L.; Giani, G.; Orlando, G.; Anselmi, M.; Gobbo, M.; Maccabruni, A.; Malfitano, A.; Cevini, C.; Bruno, A.; Maserati, R.; Giola, M.

In: Annals of Tropical Medicine and Parasitology, Vol. 96, No. 1, 2002, p. 15-18.

Research output: Contribution to journalArticle

Gatti, S, Bernuzzi, AM, Bisoffi, Z, Raglio, A, Gulletta, M, Scaglia, M, Vailati, F, Matteelli, A, Moroni, M, Visonà, R, Orani, A, Perboni, G, Volonterio, A, Galimberti, L, Giani, G, Orlando, G, Anselmi, M, Gobbo, M, Maccabruni, A, Malfitano, A, Cevini, C, Bruno, A, Maserati, R & Giola, M 2002, 'Multicentre study, in patients with imported malaria, on the sensitivity and specificity of a dipstick test (ICT Malaria P.f./P.v.™) compared with expert microscopy', Annals of Tropical Medicine and Parasitology, vol. 96, no. 1, pp. 15-18.
Gatti, S. ; Bernuzzi, A. M. ; Bisoffi, Z. ; Raglio, A. ; Gulletta, M. ; Scaglia, M. ; Vailati, F. ; Matteelli, A. ; Moroni, M. ; Visonà, R. ; Orani, A. ; Perboni, G. ; Volonterio, A. ; Galimberti, L. ; Giani, G. ; Orlando, G. ; Anselmi, M. ; Gobbo, M. ; Maccabruni, A. ; Malfitano, A. ; Cevini, C. ; Bruno, A. ; Maserati, R. ; Giola, M. / Multicentre study, in patients with imported malaria, on the sensitivity and specificity of a dipstick test (ICT Malaria P.f./P.v.™) compared with expert microscopy. In: Annals of Tropical Medicine and Parasitology. 2002 ; Vol. 96, No. 1. pp. 15-18.
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abstract = "A prospective, multicentre study was carried out in Italy to assess the sensitivity and specificity of a rapid dipstick test (ICT Malaria P.f./P.v.) in the diagnosis of imported malaria caused by Plasmodium falciparum and other Plasmodium spp. The test is based on the detection of histidine-rich protein-2 (HRP-2) from P. falciparum and 'panmalarial' antigen in peripheral blood. The 241 subjects were international travellers or immigrants from areas where malaria is endemic. When compared with the microscopical examination of bloodsmears (used as the 'gold standard'), the dipsticks were found to be 94.4{\%} sensitive and 94.5{\%} specific for pure infections with P. falciparum. The performance of the tests when used on patients infected with species other than P. falciparum or more than one Plasmodium spp. showed a high degree of variability. Although the dipsticks represent a very simple, rapid, and valuable diagnostic aid, they should not be considered a complete substitute for direct microscopical diagnosis using stained bloodsmears.",
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AU - Orlando, G.

AU - Anselmi, M.

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AU - Maccabruni, A.

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AU - Bruno, A.

AU - Maserati, R.

AU - Giola, M.

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