Multicentric evaluation of a new assay for prothrombin fragment F1+2 determination

H. D. Bruhn, J. Conard, M. Mannucci, J. Monteagudo, H. Pelzer, J. C. Reverter, M. Samama, A. Tripodi, C. Wagner

Research output: Contribution to journalArticlepeer-review

Abstract

A multicenter study of a recently developed ELISA for the determination of prothrombin fragment F1+2 was performed in order to evaluate analytical and clinical aspects. Mean intra-assay and inter-assay reproducibility were found to be 11.0 and 12.6%, respectively. The measuring range covered by the calibration curve reaches from 0.04 to 10.0nM/l F1+2. Testing 133 healthy subjects a reference range of 0.37 to 1.11nM/l F1+2 (2.5-97.5 percentile) with a median of 0.66nM/l F1+2 was calculated. Minor difficulties with blood sampling (venous occlusion for 2 min) did not affect F1+2 plasma concentrations. Significantly increased F1+2 levels were measured in patients with leukemia (p

Original languageEnglish
Pages (from-to)413-417
Number of pages5
JournalThrombosis and Haemostasis
Volume68
Issue number4
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Hematology

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