Multidifferentiation potential of human mesenchymal stem cells from adipose tissue and hamstring tendons for musculoskeletal cell-based therapy

Deborah Stanco, Marco Viganò, Carlotta Perucca Orfei, Alessia Di Giancamillo, Giuseppe M. Peretti, Luciano Lanfranchi, Laura De Girolamo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aim: Adipose-derived stem cells (ASCs) have been deeply characterized for their usefulness in musculoskeletal tissue regeneration; recently, other mesenchymal stem cell (MSC) sources have also been proposed. This study compares for the first time human tendon stem/progenitor cells isolated from hamstring tendons with human ASCs. Materials & Methods: Human TSPCs and ASCs were isolated from hamstring tendon portions and adipose tissue of healthy donors undergoing ACL reconstruction or liposuction, respectively (n = 7). Clonogenic ability, immunophenotype and multi-differentiation potential were assessed and compared. Results: Both populations showed similar proliferation and clonogenic ability and expressed embryonic stem cell genes and MSC surface markers. Tendon stem/progenitor cells showed lower adipogenic and osteogenic ability, but after the chondrogenic differentiation, they produced more abundant glycosaminoglycans and expressed higher levels of aggrecan with regards to ASCs. The tenogenic induction with BMP-12 upregulated SCX and DCN gene expression in both populations. Conclusion: Our results demonstrate that waste hamstring tendon fragments could represent a convenient MSC source for musculoskeletal regenerative medicine.

Original languageEnglish
Pages (from-to)729-743
Number of pages15
JournalRegenerative Medicine
Volume10
Issue number6
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Tendons
Cell- and Tissue-Based Therapy
Stem cells
Mesenchymal Stromal Cells
Adipose Tissue
Stem Cells
Tissue
Aggrecans
Lipectomy
Regenerative Medicine
Hamstring Tendons
Embryonic Stem Cells
Glycosaminoglycans
Tissue regeneration
Population
Regeneration
Gene expression
Tissue Donors
Gene Expression
Genes

Keywords

  • adipose-derived stem cells
  • bone morphogenetic protein-12
  • multidifferentiation potential
  • musculoskeletal pathologies
  • regenerative medicine
  • tendon
  • tendon stem progenitor cells
  • tenogenic differentiation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Cite this

Multidifferentiation potential of human mesenchymal stem cells from adipose tissue and hamstring tendons for musculoskeletal cell-based therapy. / Stanco, Deborah; Viganò, Marco; Perucca Orfei, Carlotta; Di Giancamillo, Alessia; Peretti, Giuseppe M.; Lanfranchi, Luciano; De Girolamo, Laura.

In: Regenerative Medicine, Vol. 10, No. 6, 01.09.2015, p. 729-743.

Research output: Contribution to journalArticle

Stanco, Deborah ; Viganò, Marco ; Perucca Orfei, Carlotta ; Di Giancamillo, Alessia ; Peretti, Giuseppe M. ; Lanfranchi, Luciano ; De Girolamo, Laura. / Multidifferentiation potential of human mesenchymal stem cells from adipose tissue and hamstring tendons for musculoskeletal cell-based therapy. In: Regenerative Medicine. 2015 ; Vol. 10, No. 6. pp. 729-743.
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