TY - JOUR
T1 - Multifactorial control of insulin receptor gene expression in human cell lines
AU - Briata, Paola
AU - Gherzi, Roberto
PY - 1990/8/16
Y1 - 1990/8/16
N2 - Taking advantage of a computer program to align DNA sequences we scanned the 5′-flanking region of the insulin receptor gene to localize putative regulatory elements. Using this approach as a start point, we investigated the effect of several agents on insulin receptor gene expression in human cells. We found that (i) forskolin reduces insulin receptor mRNA levels; (ii) the effects of either forskolin or dexamethasone on insulin receptor mRNA levels are near completely abolished in glucose-starved cells; (iii) 2-mercaptoethanol and heat shock, besides previously described stress conditions (glycosylatyon inhibitors), reduce insulin receptor mRNA levels.
AB - Taking advantage of a computer program to align DNA sequences we scanned the 5′-flanking region of the insulin receptor gene to localize putative regulatory elements. Using this approach as a start point, we investigated the effect of several agents on insulin receptor gene expression in human cells. We found that (i) forskolin reduces insulin receptor mRNA levels; (ii) the effects of either forskolin or dexamethasone on insulin receptor mRNA levels are near completely abolished in glucose-starved cells; (iii) 2-mercaptoethanol and heat shock, besides previously described stress conditions (glycosylatyon inhibitors), reduce insulin receptor mRNA levels.
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U2 - 10.1016/0006-291X(90)90518-R
DO - 10.1016/0006-291X(90)90518-R
M3 - Article
C2 - 1697168
AN - SCOPUS:0025131629
VL - 170
SP - 1184
EP - 1190
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -