Taking advantage of a computer program to align DNA sequences we scanned the 5′-flanking region of the insulin receptor gene to localize putative regulatory elements. Using this approach as a start point, we investigated the effect of several agents on insulin receptor gene expression in human cells. We found that (i) forskolin reduces insulin receptor mRNA levels; (ii) the effects of either forskolin or dexamethasone on insulin receptor mRNA levels are near completely abolished in glucose-starved cells; (iii) 2-mercaptoethanol and heat shock, besides previously described stress conditions (glycosylatyon inhibitors), reduce insulin receptor mRNA levels.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Aug 16 1990|
ASJC Scopus subject areas
- Molecular Biology