Multifactorial control of insulin receptor gene expression in human cell lines

Research output: Contribution to journalArticle

Abstract

Taking advantage of a computer program to align DNA sequences we scanned the 5′-flanking region of the insulin receptor gene to localize putative regulatory elements. Using this approach as a start point, we investigated the effect of several agents on insulin receptor gene expression in human cells. We found that (i) forskolin reduces insulin receptor mRNA levels; (ii) the effects of either forskolin or dexamethasone on insulin receptor mRNA levels are near completely abolished in glucose-starved cells; (iii) 2-mercaptoethanol and heat shock, besides previously described stress conditions (glycosylatyon inhibitors), reduce insulin receptor mRNA levels.

Original languageEnglish
Pages (from-to)1184-1190
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume170
Issue number3
DOIs
Publication statusPublished - Aug 16 1990

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Insulin Receptor
Gene expression
Cells
Gene Expression
Cell Line
Colforsin
Messenger RNA
Mercaptoethanol
5' Flanking Region
DNA sequences
Dexamethasone
Computer program listings
Shock
Software
Hot Temperature
Genes
Glucose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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