Multifunctionalization Modulates Hydroxyapatite Surface Interaction with Bisphosphonate: Antiosteoporotic and Antioxidative Stress Materials

Lucia Forte, Stéphanie Sarda, Paola Torricelli, Christèle Combes, Fabien Brouillet, Olivier Marsan, Francesca Salamanna, Milena Fini, Elisa Boanini, Adriana Bigi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Multifunctionalized biomaterials with enhanced bone antiresorptive properties were obtained through adsorption of a bisphosphonate, risedronate, on hydroxyapatite (HA) nanocrystals functionalized with zinc ions and polyethylenimine (PEI). Zn incorporation into the HA structure amounts to about 8 atom %, whereas the PEI content of the bifunctionalized material ZnHAPEIBP is about 5.9 wt %. The mechanism of adsorption and release of the bisphosphonate on ZnHAPEI is compared with that on ZnHA: risedronate adsorption isotherm on ZnHA is a Langmuir type, whereas the isotherm of adsorption on ZnHAPEI is better fitted with a Freundlich model and involved a higher amount of adsorbed risedronate. In vitro cell tests were carried out with a coculture model of osteoblasts and osteoclasts using a model simulating oxidative stress and consequent cellular senescence and osteoporosis by the addition of H2O2. The conditions utilized in the coculture model strongly affect osteoblast behavior. The results show that the composite materials allow an increase in osteoblast viability and recover impairment, revealing a novel characteristic of risedronate that is able to counteract the negative effects of oxidative stress when associated with differently functionalized samples. Both PEI and the bisphosphonate reduce osteoclast viability. Moreover, PEI, and even more risedronate, exerts an inhibitory effect on osteoclast activity.

Original languageEnglish
Pages (from-to)3429-3439
Number of pages11
JournalACS Biomaterials Science & Engineering
DOIs
Publication statusPublished - Jun 11 2019

Fingerprint

Diphosphonates
Durapatite
Polyethyleneimine
Hydroxyapatite
Osteoblasts
Oxidative stress
Adsorption
Adsorption isotherms
Biomaterials
Nanocrystals
Isotherms
Biocompatible Materials
Bone
Zinc
Atoms
Risedronate Sodium
Ions
Composite materials
Osteoclasts

Keywords

  • adsorption
  • bisphosphonate
  • hydroxyapatite
  • osteoporosis
  • oxidative stress
  • polyethylenimine
  • zinc

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Multifunctionalization Modulates Hydroxyapatite Surface Interaction with Bisphosphonate: Antiosteoporotic and Antioxidative Stress Materials. / Forte, Lucia; Sarda, Stéphanie; Torricelli, Paola; Combes, Christèle; Brouillet, Fabien; Marsan, Olivier; Salamanna, Francesca; Fini, Milena; Boanini, Elisa; Bigi, Adriana.

In: ACS Biomaterials Science & Engineering, 11.06.2019, p. 3429-3439.

Research output: Contribution to journalArticle

Forte, Lucia ; Sarda, Stéphanie ; Torricelli, Paola ; Combes, Christèle ; Brouillet, Fabien ; Marsan, Olivier ; Salamanna, Francesca ; Fini, Milena ; Boanini, Elisa ; Bigi, Adriana. / Multifunctionalization Modulates Hydroxyapatite Surface Interaction with Bisphosphonate: Antiosteoporotic and Antioxidative Stress Materials. In: ACS Biomaterials Science & Engineering. 2019 ; pp. 3429-3439.
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AU - Brouillet, Fabien

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