Abstract
Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 + cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.
| Original language | English |
|---|---|
| Pages (from-to) | 369-372 |
| Number of pages | 4 |
| Journal | Haematologica |
| Volume | 91 |
| Issue number | 3 |
| Publication status | Published - Mar 2006 |
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Keywords
- Cord blood transplantation
- Cryopreservation
- Refreezing
ASJC Scopus subject areas
- Hematology
Cite this
Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice. / Timeus, Fabio; Crescenzio, Nicoletta; Sanavio, Fiorella; Fazio, Luigina; Doria, Alessandra; Foglia, Luiseida; Pignochino, Ymera; Berger, Massimo; Piacibello, Wanda; Madon, Enrico; Di Montezemolo, Luca Cordero; Fagioli, Franca.
In: Haematologica, Vol. 91, No. 3, 03.2006, p. 369-372.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice
AU - Timeus, Fabio
AU - Crescenzio, Nicoletta
AU - Sanavio, Fiorella
AU - Fazio, Luigina
AU - Doria, Alessandra
AU - Foglia, Luiseida
AU - Pignochino, Ymera
AU - Berger, Massimo
AU - Piacibello, Wanda
AU - Madon, Enrico
AU - Di Montezemolo, Luca Cordero
AU - Fagioli, Franca
PY - 2006/3
Y1 - 2006/3
N2 - Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 + cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.
AB - Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 + cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.
KW - Cord blood transplantation
KW - Cryopreservation
KW - Refreezing
UR - http://www.scopus.com/inward/record.url?scp=33645415464&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645415464&partnerID=8YFLogxK
M3 - Article
C2 - 16531260
AN - SCOPUS:33645415464
VL - 91
SP - 369
EP - 372
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 3
ER -