Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice

Fabio Timeus; Nicoletta Crescenzio; Fiorella Sanavio; Luigina Fazio; Alessandra Doria; Luiseida Foglia; Ymera Pignochino; Massimo Berger; Wanda Piacibello; Enrico Madon; Luca Cordero Di Montezemolo; Franca Fagioli

Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice

Fabio Timeus, Nicoletta Crescenzio, Fiorella Sanavio, Luigina Fazio, Alessandra Doria, Luiseida Foglia, Ymera Pignochino, Massimo Berger, Wanda Piacibello, Enrico Madon, Luca Cordero Di Montezemolo, Franca Fagioli

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 ⁺ cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.

Original language	English
Pages (from-to)	369-372
Number of pages	4
Journal	Haematologica
Volume	91
Issue number	3
Publication status	Published - Mar 2006

Keywords

Cord blood transplantation
Cryopreservation
Refreezing

ASJC Scopus subject areas

Hematology

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Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice. / Timeus, Fabio; Crescenzio, Nicoletta; Sanavio, Fiorella; Fazio, Luigina; Doria, Alessandra; Foglia, Luiseida; Pignochino, Ymera; Berger, Massimo; Piacibello, Wanda; Madon, Enrico; Di Montezemolo, Luca Cordero; Fagioli, Franca.

In: Haematologica, Vol. 91, No. 3, 03.2006, p. 369-372.

Research output: Contribution to journal › Article › peer-review

Timeus, Fabio ; Crescenzio, Nicoletta ; Sanavio, Fiorella ; Fazio, Luigina ; Doria, Alessandra ; Foglia, Luiseida ; Pignochino, Ymera ; Berger, Massimo ; Piacibello, Wanda ; Madon, Enrico ; Di Montezemolo, Luca Cordero ; Fagioli, Franca. / Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice. In: Haematologica. 2006 ; Vol. 91, No. 3. pp. 369-372.

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abstract = "Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 + cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.",

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author = "Fabio Timeus and Nicoletta Crescenzio and Fiorella Sanavio and Luigina Fazio and Alessandra Doria and Luiseida Foglia and Ymera Pignochino and Massimo Berger and Wanda Piacibello and Enrico Madon and {Di Montezemolo}, {Luca Cordero} and Franca Fagioli",

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AU - Timeus, Fabio

AU - Crescenzio, Nicoletta

AU - Sanavio, Fiorella

AU - Fazio, Luigina

AU - Doria, Alessandra

AU - Foglia, Luiseida

AU - Pignochino, Ymera

AU - Berger, Massimo

AU - Piacibello, Wanda

AU - Madon, Enrico

AU - Di Montezemolo, Luca Cordero

AU - Fagioli, Franca

PY - 2006/3

Y1 - 2006/3

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AB - Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 + cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.

KW - Cord blood transplantation

KW - Cryopreservation

KW - Refreezing

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Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice

Abstract

Keywords

ASJC Scopus subject areas

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