Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice

Fabio Timeus, Nicoletta Crescenzio, Fiorella Sanavio, Luigina Fazio, Alessandra Doria, Luiseida Foglia, Ymera Pignochino, Massimo Berger, Wanda Piacibello, Enrico Madon, Luca Cordero Di Montezemolo, Franca Fagioli

Research output: Contribution to journalArticle

Abstract

Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45 + cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the α satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.

Original languageEnglish
Pages (from-to)369-372
Number of pages4
JournalHaematologica
Volume91
Issue number3
Publication statusPublished - Mar 2006

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Keywords

  • Cord blood transplantation
  • Cryopreservation
  • Refreezing

ASJC Scopus subject areas

  • Hematology

Cite this

Timeus, F., Crescenzio, N., Sanavio, F., Fazio, L., Doria, A., Foglia, L., Pignochino, Y., Berger, M., Piacibello, W., Madon, E., Di Montezemolo, L. C., & Fagioli, F. (2006). Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice. Haematologica, 91(3), 369-372.