Multimeric pattern of plasma and platelet von Willebrand factor is normal in uremic patients

G. Castaman, F. Rodeghiero, A. Lattuada, G. La Greca, P. M. Mannucci

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Several studies have reported increased von Willebrand factor (vWF) levels in plasma of uremic patients, with a normal multimeric pattern. Two recent studies, however, have shown a reduction of the larger multimers of plasma vWF and one has also found low levels of platelet vWF in uremic patients with prolonged bleeding time (BT). We have measured plasma and platelet vWF and analyzed its multimeric pattern in 20 uremic patients, 11 with a prolonged BT and 9 with a normal BT. For Plasma vWF, no difference for vWF:Ag, RiCof, and ristocetin-induced platelet agglutination was found between the two groups with normal and prolonged BT. The multimeric pattern of plasma vWF, as evaluated by densitometric scanning of the electrophoretic gels, was normal in both groups. For platelet vWF, vWF:Ag and RiCof content was similar in the two groups. The multimeric pattern was indistinguishable from that of normal platelets. In conclusion, our study does not confirm the presence of a structural defect of plasma vWF and the reduction of platelet vWF content in uremia.

Original languageEnglish
Pages (from-to)266-269
Number of pages4
JournalAmerican Journal of Hematology
Volume44
Issue number4
DOIs
Publication statusPublished - 1993

Fingerprint

von Willebrand Factor
Blood Platelets
Bleeding Time
Ristocetin
Uremia
Agglutination
Gels

Keywords

  • acquired bleeding diathesis
  • bleeding time
  • uremia
  • von Willebrand factor

ASJC Scopus subject areas

  • Hematology

Cite this

Multimeric pattern of plasma and platelet von Willebrand factor is normal in uremic patients. / Castaman, G.; Rodeghiero, F.; Lattuada, A.; La Greca, G.; Mannucci, P. M.

In: American Journal of Hematology, Vol. 44, No. 4, 1993, p. 266-269.

Research output: Contribution to journalArticle

Castaman, G. ; Rodeghiero, F. ; Lattuada, A. ; La Greca, G. ; Mannucci, P. M. / Multimeric pattern of plasma and platelet von Willebrand factor is normal in uremic patients. In: American Journal of Hematology. 1993 ; Vol. 44, No. 4. pp. 266-269.
@article{ce6f857e030e44bd83e97ad1b1e87200,
title = "Multimeric pattern of plasma and platelet von Willebrand factor is normal in uremic patients",
abstract = "Several studies have reported increased von Willebrand factor (vWF) levels in plasma of uremic patients, with a normal multimeric pattern. Two recent studies, however, have shown a reduction of the larger multimers of plasma vWF and one has also found low levels of platelet vWF in uremic patients with prolonged bleeding time (BT). We have measured plasma and platelet vWF and analyzed its multimeric pattern in 20 uremic patients, 11 with a prolonged BT and 9 with a normal BT. For Plasma vWF, no difference for vWF:Ag, RiCof, and ristocetin-induced platelet agglutination was found between the two groups with normal and prolonged BT. The multimeric pattern of plasma vWF, as evaluated by densitometric scanning of the electrophoretic gels, was normal in both groups. For platelet vWF, vWF:Ag and RiCof content was similar in the two groups. The multimeric pattern was indistinguishable from that of normal platelets. In conclusion, our study does not confirm the presence of a structural defect of plasma vWF and the reduction of platelet vWF content in uremia.",
keywords = "acquired bleeding diathesis, bleeding time, uremia, von Willebrand factor",
author = "G. Castaman and F. Rodeghiero and A. Lattuada and {La Greca}, G. and Mannucci, {P. M.}",
year = "1993",
doi = "10.1002/ajh.2830440409",
language = "English",
volume = "44",
pages = "266--269",
journal = "American Journal of Hematology",
issn = "0361-8609",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Multimeric pattern of plasma and platelet von Willebrand factor is normal in uremic patients

AU - Castaman, G.

AU - Rodeghiero, F.

AU - Lattuada, A.

AU - La Greca, G.

AU - Mannucci, P. M.

PY - 1993

Y1 - 1993

N2 - Several studies have reported increased von Willebrand factor (vWF) levels in plasma of uremic patients, with a normal multimeric pattern. Two recent studies, however, have shown a reduction of the larger multimers of plasma vWF and one has also found low levels of platelet vWF in uremic patients with prolonged bleeding time (BT). We have measured plasma and platelet vWF and analyzed its multimeric pattern in 20 uremic patients, 11 with a prolonged BT and 9 with a normal BT. For Plasma vWF, no difference for vWF:Ag, RiCof, and ristocetin-induced platelet agglutination was found between the two groups with normal and prolonged BT. The multimeric pattern of plasma vWF, as evaluated by densitometric scanning of the electrophoretic gels, was normal in both groups. For platelet vWF, vWF:Ag and RiCof content was similar in the two groups. The multimeric pattern was indistinguishable from that of normal platelets. In conclusion, our study does not confirm the presence of a structural defect of plasma vWF and the reduction of platelet vWF content in uremia.

AB - Several studies have reported increased von Willebrand factor (vWF) levels in plasma of uremic patients, with a normal multimeric pattern. Two recent studies, however, have shown a reduction of the larger multimers of plasma vWF and one has also found low levels of platelet vWF in uremic patients with prolonged bleeding time (BT). We have measured plasma and platelet vWF and analyzed its multimeric pattern in 20 uremic patients, 11 with a prolonged BT and 9 with a normal BT. For Plasma vWF, no difference for vWF:Ag, RiCof, and ristocetin-induced platelet agglutination was found between the two groups with normal and prolonged BT. The multimeric pattern of plasma vWF, as evaluated by densitometric scanning of the electrophoretic gels, was normal in both groups. For platelet vWF, vWF:Ag and RiCof content was similar in the two groups. The multimeric pattern was indistinguishable from that of normal platelets. In conclusion, our study does not confirm the presence of a structural defect of plasma vWF and the reduction of platelet vWF content in uremia.

KW - acquired bleeding diathesis

KW - bleeding time

KW - uremia

KW - von Willebrand factor

UR - http://www.scopus.com/inward/record.url?scp=0027374631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027374631&partnerID=8YFLogxK

U2 - 10.1002/ajh.2830440409

DO - 10.1002/ajh.2830440409

M3 - Article

C2 - 8237998

AN - SCOPUS:0027374631

VL - 44

SP - 266

EP - 269

JO - American Journal of Hematology

JF - American Journal of Hematology

SN - 0361-8609

IS - 4

ER -