Multimodal-3D imaging based on μMRI and μCT techniques bridges the gap with histology in visualization of the bone regeneration process

R. Sinibaldi, A. Conti, B. Sinjari, S. Spadone, R. Pecci, M. Palombo, V. S. Komlev, M. G. Ortore, G. Tromba, S. Capuani, R. Guidotti, F. De Luca, S. Caputi, T. Traini, S. Della Penna

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bone repair/regeneration is usually investigated through X-ray computed microtomography (μCT) supported by histology of extracted samples, to analyse biomaterial structure and new bone formation processes. Magnetic resonance imaging (μMRI) shows a richer tissue contrast than μCT, despite at lower resolution, and could be combined with μCT in the perspective of conducting non-destructive 3D investigations of bone. A pipeline designed to combine μMRI and μCT images of bone samples is here described and applied on samples of extracted human jawbone core following bone graft. We optimized the coregistration procedure between μCT and μMRI images to avoid bias due to the different resolutions and contrasts. Furthermore, we used an Adaptive Multivariate Clustering, grouping homologous voxels in the coregistered images, to visualize different tissue types within a fused 3D metastructure. The tissue grouping matched the 2D histology applied only on 1 slice, thus extending the histology labelling in 3D. Specifically, in all samples, we could separate and map 2 types of regenerated bone, calcified tissue, soft tissues, and/or fat and marrow space. Remarkably, μMRI and μCT alone were not able to separate the 2 types of regenerated bone. Finally, we computed volumes of each tissue in the 3D metastructures, which might be exploited by quantitative simulation. The 3D metastructure obtained through our pipeline represents a first step to bridge the gap between the quality of information obtained from 2D optical microscopy and the 3D mapping of the bone tissue heterogeneity and could allow researchers and clinicians to non-destructively characterize and follow-up bone regeneration.

Original languageEnglish
Pages (from-to)750-761
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

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Multimodal Imaging
Bone Regeneration
Histology
Magnetic resonance imaging
Bone
Visualization
Magnetic Resonance Imaging
Imaging techniques
Bone and Bones
Tissue
X-Ray Microtomography
Biocompatible Materials
Pipelines
Osteogenesis
Cluster Analysis
Microscopy
Bone Marrow
Fats
Research Personnel
Oils and fats

Keywords

  • 3D mapping
  • bone regeneration
  • image coregistration
  • micro magnetic resonance imaging
  • multimodal imaging
  • multivariate clustering
  • X-ray computed microtomography

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Multimodal-3D imaging based on μMRI and μCT techniques bridges the gap with histology in visualization of the bone regeneration process. / Sinibaldi, R.; Conti, A.; Sinjari, B.; Spadone, S.; Pecci, R.; Palombo, M.; Komlev, V. S.; Ortore, M. G.; Tromba, G.; Capuani, S.; Guidotti, R.; De Luca, F.; Caputi, S.; Traini, T.; Della Penna, S.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 3, 01.03.2018, p. 750-761.

Research output: Contribution to journalArticle

Sinibaldi, R, Conti, A, Sinjari, B, Spadone, S, Pecci, R, Palombo, M, Komlev, VS, Ortore, MG, Tromba, G, Capuani, S, Guidotti, R, De Luca, F, Caputi, S, Traini, T & Della Penna, S 2018, 'Multimodal-3D imaging based on μMRI and μCT techniques bridges the gap with histology in visualization of the bone regeneration process', Journal of Tissue Engineering and Regenerative Medicine, vol. 12, no. 3, pp. 750-761. https://doi.org/10.1002/term.2494
Sinibaldi, R. ; Conti, A. ; Sinjari, B. ; Spadone, S. ; Pecci, R. ; Palombo, M. ; Komlev, V. S. ; Ortore, M. G. ; Tromba, G. ; Capuani, S. ; Guidotti, R. ; De Luca, F. ; Caputi, S. ; Traini, T. ; Della Penna, S. / Multimodal-3D imaging based on μMRI and μCT techniques bridges the gap with histology in visualization of the bone regeneration process. In: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Vol. 12, No. 3. pp. 750-761.
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abstract = "Bone repair/regeneration is usually investigated through X-ray computed microtomography (μCT) supported by histology of extracted samples, to analyse biomaterial structure and new bone formation processes. Magnetic resonance imaging (μMRI) shows a richer tissue contrast than μCT, despite at lower resolution, and could be combined with μCT in the perspective of conducting non-destructive 3D investigations of bone. A pipeline designed to combine μMRI and μCT images of bone samples is here described and applied on samples of extracted human jawbone core following bone graft. We optimized the coregistration procedure between μCT and μMRI images to avoid bias due to the different resolutions and contrasts. Furthermore, we used an Adaptive Multivariate Clustering, grouping homologous voxels in the coregistered images, to visualize different tissue types within a fused 3D metastructure. The tissue grouping matched the 2D histology applied only on 1 slice, thus extending the histology labelling in 3D. Specifically, in all samples, we could separate and map 2 types of regenerated bone, calcified tissue, soft tissues, and/or fat and marrow space. Remarkably, μMRI and μCT alone were not able to separate the 2 types of regenerated bone. Finally, we computed volumes of each tissue in the 3D metastructures, which might be exploited by quantitative simulation. The 3D metastructure obtained through our pipeline represents a first step to bridge the gap between the quality of information obtained from 2D optical microscopy and the 3D mapping of the bone tissue heterogeneity and could allow researchers and clinicians to non-destructively characterize and follow-up bone regeneration.",
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AU - Capuani, S.

AU - Guidotti, R.

AU - De Luca, F.

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