Multiparameter flow cytometry characterization of MHC class I negative mouse bone marrow cells

Mattia Quarta, Deborah Stroka, Adrian Keogh, Daniel Sidler, Itzhak Avital, Beat Gloor, Maurizio Muraca, Daniel Candinas, Daniel Inderbitzin

Research output: Contribution to journalArticle

Abstract

Background: MHC-I down-regulation was described in foetal liver progenitors, and two different subsets of adult bone marrow derived stem cells. These cells, namely, MHC-I-/Thy1+ bone marrow derived liver stem cells (BMDLSC) and the multipotent adult progenitors (MAPC) differentiated into functioning hepatocytes. The aim of this paper was to characterize the MHC-I negative bone marrow compartment as it pertains to BMDLSC and MAPC. Material/Methods: We performed multiparameter flow-cytometry analyses of the MHC-I negative compartment using hematopoietic (CD45, Ter119), and stem cell markers (Thy1.2, c-Kit, IL-3R, CD34) in adult mice. Results: When analysing CD45 and Ter119 expression, the MHC-I negative bone marrow compartment divides into four sub-populations: 1. CD45-/Ter119+: 86.0±4.4%; 2. CD45+/Ter119+: 0.2±0.1%; 3. CD45+/Ter119-: 11.6±3.0%; 4. CD45 -/Ter119-: 2.0±2.1%. Stem cells markers were only expressed on MHC-I negative/ CD45+/Ter119- cells. In vivo, MAPC (Ter119-/CD45- cells) are composed of MHC-I negative (24%) and MHC-I positive cells and do not express any of the stem cell markers tested. Conclusions: In conclusion, mouse BMDLSC and MAPC are two distinct stem cell populations. Down-regulation of MHC-I was the only common characteristic found between BMDLSC and MAPC suggesting that selection of MHC-I negative cells might represent an efficient strategy to enrich for bone marrow stem cells with liver developmental potential.

Original languageEnglish
JournalMedical Science Monitor
Volume14
Issue number12
Publication statusPublished - Dec 2008

Keywords

  • Bone marrow
  • Major histocompatibility complex class I
  • Multiparameter flow cytometry
  • Rodent
  • Stem cells

ASJC Scopus subject areas

  • Medicine(all)

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