Multiple binding reactivities of an IgG1 mouse monoclonal antibody raised against the extracellular matrix glycoprotein Gp 115.

A. Colombatti, G. M. Bressan, P. Bonaldo, I. Castellani, D. Volpin

Research output: Contribution to journalArticlepeer-review

Abstract

An IgG1 mouse monoclonal antibody that recognized five different polypeptides from chick and man is described. The antibody was raised against the elastin associated chick glycoprotein gp 115. In addition to the immunizing antigen, antibody 106D6 bound to chick smooth muscle cell myosin and to the alpha, beta and gamma chains of human fibrinogen. Cross reaction was demonstrated by radioimmunobinding, immunofluorescence and immunoblotting experiments. The immunoblotting assays were carried out using identical amounts of the various antigens to exclude the possibility that relevant quantitative differences in their concentration might result in non-specific binding.

Original languageEnglish
Pages (from-to)349-358
Number of pages10
JournalHybridoma
Volume6
Issue number4
Publication statusPublished - Aug 1987

ASJC Scopus subject areas

  • Genetics
  • Immunology

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