Multiple localization of endogenous MARK4L protein in human glioma

Ivana Magnani, Chiara Novielli, Melissa Bellini, Gaia Roversi, Lorenzo Bello, Lidia Larizza

Research output: Contribution to journalArticle

Abstract

Background: We have previously shown that the sustained expression of MARK4L transcripts in glioma and neural progenitors (NHNPs) declines after exposure to antisense MARK4L oligonucleotides in glioblastoma cell lines. Array-CGH confirmed the genomic duplication of MARK4L identified by FISH in a glioblastoma cell line. This background together with literature data on the exogenous association of MARK4 with interphase centrosome prompted us to investigate the sub-cellular localization of the endogenous MARK4L protein aiming at achieving insights on its possible role in the pathomechanisms of glioma. Methods: Immunodetection was carried out to validate the specificity of MARK4L antibody in gliomas and NHNPs. Mass spectrometry was applied for MARK4L protein identification in a representative glioblastoma cell line. Combined biochemical fractionation and immunodetection analyses were performed to confirm the sub-cellular localization of MARK4L achieved by immunofluorescence in glioma cell lines. Results: By assigning MARK4L protein within the band immunoprecipitated by the specific antibody we validated our antiMARK4L antibody. We demonstrated that the endogenous MARK4L: (i) colocalizes with centrosomes at all mitotic stages and resides in centrosome-enriched fractions; (ii) associates with the nucleolus and the midbody and respective fractions, and (iii) co-stains the aberrant centrosome configurations observed in glioma cell lines. Conclusion: The overall data merge on the multiplex entry of MARK4L into the cell cycle and link it to the aberrant centrosomes in glioma cell lines suggesting a possible role of this kinase in the abnormal mitotic processes of human glioma.

Original languageEnglish
Pages (from-to)357-370
Number of pages14
JournalCellular Oncology
Volume31
Issue number5
DOIs
Publication statusPublished - 2009

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Keywords

  • Centrosome abnormalities
  • Glioma
  • Immunodetection
  • MARK4L
  • Multiple subcellular localization

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine

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