Multiple malaria infection in a pregnant woman from Nigeria: Detection by multiplex PCR

Rosalia Graffeo, Luca Masucci, Francesca Bugli, Fernando Damiano, Carmen Pinnetti, Giovanni Federico, Giovanni Fadda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

For the last 100 years, diagnosis of malaria has been based on examination of Giemsa-stained thick and thin blood films under the microscope. This is a time-consuming procedure which often fails to correctly diagnose the infecting species - especially when carried out by inexperienced technicians or when blood levels of parasite are low. Rapid Diagnostic Tests (RDTs) for antigen detection can distinguish between Plasmodium falciparum and Plasmodium vivax but cannot identify the species present in mixed infections. In the case reported here we used multiplex PCR to investigate suspected mixed infection in a pregnant woman from Nigeria. The results suggest that the method used is highly specific and can be very sensitive and that it has several advantages with respect to microscopy and RTDs.

Original languageEnglish
Pages (from-to)565-567
Number of pages3
JournalNew Microbiologica
Volume31
Issue number4
Publication statusPublished - Oct 2008

Fingerprint

Multiplex Polymerase Chain Reaction
Nigeria
Coinfection
Malaria
Pregnant Women
Plasmodium vivax
Plasmodium falciparum
Infection
Routine Diagnostic Tests
Microscopy
Parasites
Antigens

Keywords

  • Molecular detection
  • Plasmodium

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Graffeo, R., Masucci, L., Bugli, F., Damiano, F., Pinnetti, C., Federico, G., & Fadda, G. (2008). Multiple malaria infection in a pregnant woman from Nigeria: Detection by multiplex PCR. New Microbiologica, 31(4), 565-567.

Multiple malaria infection in a pregnant woman from Nigeria : Detection by multiplex PCR. / Graffeo, Rosalia; Masucci, Luca; Bugli, Francesca; Damiano, Fernando; Pinnetti, Carmen; Federico, Giovanni; Fadda, Giovanni.

In: New Microbiologica, Vol. 31, No. 4, 10.2008, p. 565-567.

Research output: Contribution to journalArticle

Graffeo, R, Masucci, L, Bugli, F, Damiano, F, Pinnetti, C, Federico, G & Fadda, G 2008, 'Multiple malaria infection in a pregnant woman from Nigeria: Detection by multiplex PCR', New Microbiologica, vol. 31, no. 4, pp. 565-567.
Graffeo R, Masucci L, Bugli F, Damiano F, Pinnetti C, Federico G et al. Multiple malaria infection in a pregnant woman from Nigeria: Detection by multiplex PCR. New Microbiologica. 2008 Oct;31(4):565-567.
Graffeo, Rosalia ; Masucci, Luca ; Bugli, Francesca ; Damiano, Fernando ; Pinnetti, Carmen ; Federico, Giovanni ; Fadda, Giovanni. / Multiple malaria infection in a pregnant woman from Nigeria : Detection by multiplex PCR. In: New Microbiologica. 2008 ; Vol. 31, No. 4. pp. 565-567.
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