Multiple receptors mediate the trophic effects of serotonin on ventroposterior thalamic neurons in vitro

Antonio M. Persico, Giovanni Di Pino, Pat Levitt

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Serotonin (5-HT) exerts prominent morphogenetic roles during development. For example, somatosensory cortical barrel formation is altered in mouse models characterized by excessive extracellular 5-HT, suggesting that 5-HT affects development of thalamic afferents and/or neocortical target regions. The present study assessed 5-HT effects in primary cultures of fetal ventroposterior thalamic (VPT) neurons. 5-HT produces concentration-dependent trophic effects, with impressive 59% and 106% peak increases in total neurite length and number of branching points, respectively, at a dose of 30 μM 5-HT. The exposure of VPT neurons to specific 5-HT receptor agonists 8-OH-DPAT (5-HT1A), CGS-12066A (5-HT1B), DOI (5-HT2A/2C), and m-CPBG (5-HT3), enhances primary neurite length and number of branching points with rank-order potency 5-HT1B > 5-HT2A/2C = 5-HT 3 > 5-HT1A = vehicle. Trophic 5-HT effects on embryonic VPT neurons are thus much more prominent than previously reported, and can be mediated by multiple 5-HT receptor subtypes.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalBrain Research
Volume1095
Issue number1
DOIs
Publication statusPublished - 2006

Fingerprint

Serotonin
Neurons
Serotonin Receptors
Neurites
In Vitro Techniques
8-Hydroxy-2-(di-n-propylamino)tetralin
Serotonin Receptor Agonists

Keywords

  • 5-HT receptor
  • 5-hydroxytryptamine
  • Barrel
  • Neurite outgrowth
  • Serotonin
  • Somatosensory system
  • Thalamus
  • Tissue culture

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Multiple receptors mediate the trophic effects of serotonin on ventroposterior thalamic neurons in vitro. / Persico, Antonio M.; Di Pino, Giovanni; Levitt, Pat.

In: Brain Research, Vol. 1095, No. 1, 2006, p. 17-25.

Research output: Contribution to journalArticle

Persico, Antonio M. ; Di Pino, Giovanni ; Levitt, Pat. / Multiple receptors mediate the trophic effects of serotonin on ventroposterior thalamic neurons in vitro. In: Brain Research. 2006 ; Vol. 1095, No. 1. pp. 17-25.
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