Multiplex PCR approach to simultaneously identify several mutations in fine needle cytology thyroid samples

Emilia Vuttariello, Marco Borra, Elvira Mauriello, Celeste Calise, Barbara D'Andrea, Anna Capiluongo, Franco Fulciniti, Anna Cipolletta, Mario Monaco, Luciano Pezzullo, Gennaro Chiappetta

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The most frequent initial manifestation of thyroid cancer is the appearance of a nodule. More than 20% of the general population has a palpable thyroid nodule and the percentage rises to 70% based on ultrasound identification. In 95% of cases the nodule is simply a hyperplastic or benign lesion. The most reliable diagnostic test for thyroid nodules is fine needle aspiration (FNA), but cytological discrimination between malignant and benign follicular neoplasms remains difficult. Cytological analysis is now, almost routinely, being combined with molecular genetics to enable the pathologist to make a more objective diagnosis. In this study, we performed the molecular analysis using a new simplified procedure that involves a panel of BRAF, RAS, RET and RET/PTC gene mutations in easily obtainable FNA samples, in the attempt to improve the efficacy of the FNA diagnosis of thyroid nodules and thus patient management. In this new procedure, PCR and sequencing analysis are used to detect point mutations, and, in parallel, RT-PCR is used to detect the chimeric RET/PTC1 and RET/PTC3 transcripts in RNA extracted from FNA.

Original languageEnglish
JournalOncotarget
DOIs
Publication statusPublished - Jul 2017

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Multiplex Polymerase Chain Reaction
Fine Needle Biopsy
Thyroid Nodule
Needles
Cell Biology
Thyroid Gland
Mutation
Polymerase Chain Reaction
Factor IX
Thyroid Neoplasms
Point Mutation
Routine Diagnostic Tests
Molecular Biology
RNA
Population
Genes
Neoplasms

Keywords

  • Journal Article

Cite this

Multiplex PCR approach to simultaneously identify several mutations in fine needle cytology thyroid samples. / Vuttariello, Emilia; Borra, Marco; Mauriello, Elvira; Calise, Celeste; D'Andrea, Barbara; Capiluongo, Anna; Fulciniti, Franco; Cipolletta, Anna; Monaco, Mario; Pezzullo, Luciano; Chiappetta, Gennaro.

In: Oncotarget, 07.2017.

Research output: Contribution to journalArticle

Vuttariello, Emilia ; Borra, Marco ; Mauriello, Elvira ; Calise, Celeste ; D'Andrea, Barbara ; Capiluongo, Anna ; Fulciniti, Franco ; Cipolletta, Anna ; Monaco, Mario ; Pezzullo, Luciano ; Chiappetta, Gennaro. / Multiplex PCR approach to simultaneously identify several mutations in fine needle cytology thyroid samples. In: Oncotarget. 2017.
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AU - Borra, Marco

AU - Mauriello, Elvira

AU - Calise, Celeste

AU - D'Andrea, Barbara

AU - Capiluongo, Anna

AU - Fulciniti, Franco

AU - Cipolletta, Anna

AU - Monaco, Mario

AU - Pezzullo, Luciano

AU - Chiappetta, Gennaro

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