Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy

Julia D. Wulfkuhle, Runa Speer, Mariaelena Pierobon, Julie Laird, Virginia Espina, Jianghong Deng, Enzo Mammano, Herry X. Yang, Sandra M. Swain, Donato Nitti, Laura J. Esserman, Claudio Belluco, Lance A. Liotta, Emanuel F. Petricoin

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.

Original languageEnglish
Pages (from-to)1508-1517
Number of pages10
JournalJournal of Proteome Research
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 2008

Keywords

  • Breast cancer
  • Cell signaling
  • Phosphoproteomics
  • Protein microarray
  • Proteomics
  • Tailored therapy

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Genetics
  • Biotechnology

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