Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors

Paola Goldoni, Lorena Cattani, Stefania Carrara, Maddalena Castellani Pastoris, Laura Sinibaldi, Nicola Orsi

Research output: Contribution to journalArticle

Abstract

A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6. The effects of the substances were separately tested on both penetration and intracellular multiplication of L pneumophila. Only cytochalasin A and 2-deoxy-D-glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalMicrobiology and Immunology
Volume42
Issue number4
Publication statusPublished - 1998

Fingerprint

Legionella pneumophila
Deoxyglucose
Cytoskeleton
HeLa Cells
Cytochalasin D
Legionella
Cytochalasin B
Microtubules
Glucose
cytochalasin A

Keywords

  • Cytoskeletal inhibitor
  • Intracellular multiplication
  • Legionella pneumophila
  • Metabolic inhibitor

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors. / Goldoni, Paola; Cattani, Lorena; Carrara, Stefania; Pastoris, Maddalena Castellani; Sinibaldi, Laura; Orsi, Nicola.

In: Microbiology and Immunology, Vol. 42, No. 4, 1998, p. 271-279.

Research output: Contribution to journalArticle

Goldoni, P, Cattani, L, Carrara, S, Pastoris, MC, Sinibaldi, L & Orsi, N 1998, 'Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors', Microbiology and Immunology, vol. 42, no. 4, pp. 271-279.
Goldoni, Paola ; Cattani, Lorena ; Carrara, Stefania ; Pastoris, Maddalena Castellani ; Sinibaldi, Laura ; Orsi, Nicola. / Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors. In: Microbiology and Immunology. 1998 ; Vol. 42, No. 4. pp. 271-279.
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