Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors

Paola Goldoni, Lorena Cattani, Stefania Carrara, Maddalena Castellani Pastoris, Laura Sinibaldi, Nicola Orsi

Research output: Contribution to journalArticlepeer-review

Abstract

A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6. The effects of the substances were separately tested on both penetration and intracellular multiplication of L pneumophila. Only cytochalasin A and 2-deoxy-D-glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalMicrobiology and Immunology
Volume42
Issue number4
Publication statusPublished - 1998

Keywords

  • Cytoskeletal inhibitor
  • Intracellular multiplication
  • Legionella pneumophila
  • Metabolic inhibitor

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

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