Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo

Romain Zufferey, Dea Nagy, Ron J. Mandel, Luigi Naldini, Didier Trono

Research output: Contribution to journalArticle

1395 Citations (Scopus)

Abstract

Retroviral vectors derived from lentiviruses such as HIV-1 are promising tools for human gene therapy because they mediate the in vive delivery and long-term expression of transgenes in nondividing tissues. We describe an HIV vector system in which the virulence genes env, vif, vpr, vpu, and nef have been deleted. This multiply attenuated vector conserved the ability to transduce growth-arrested cells and monocyte-derived macrophages in culture, and could efficiently deliver genes in vive into adult neurons. These data demonstrate the potential of lentiviral vectors in human gene therapy.

Original languageEnglish
Pages (from-to)871-875
Number of pages5
JournalNature Biotechnology
Volume15
Issue number9
Publication statusPublished - Sep 1997

Fingerprint

Genetic Therapy
Genes
vif Genes
env Genes
Gene therapy
Lentivirus
Transgenes
Virulence
HIV-1
Macrophages
HIV
Neurons
Cell growth
Growth
Cell culture
Tissue

Keywords

  • Gene therapy
  • HIV
  • Retrovital vectors

ASJC Scopus subject areas

  • Microbiology

Cite this

Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nature Biotechnology, 15(9), 871-875.

Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. / Zufferey, Romain; Nagy, Dea; Mandel, Ron J.; Naldini, Luigi; Trono, Didier.

In: Nature Biotechnology, Vol. 15, No. 9, 09.1997, p. 871-875.

Research output: Contribution to journalArticle

Zufferey, R, Nagy, D, Mandel, RJ, Naldini, L & Trono, D 1997, 'Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo', Nature Biotechnology, vol. 15, no. 9, pp. 871-875.
Zufferey, Romain ; Nagy, Dea ; Mandel, Ron J. ; Naldini, Luigi ; Trono, Didier. / Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. In: Nature Biotechnology. 1997 ; Vol. 15, No. 9. pp. 871-875.
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