MULTIPRINS: a method for multicolour primed in situ labelling

E. V. Volpi, A. Baldini

Research output: Contribution to journalArticle

Abstract

With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalChromosome Research
Volume1
Issue number4
DOIs
Publication statusPublished - Nov 1993

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Keywords

  • fluorescence labelling
  • MULTIPRINS
  • repetitive DNA mapping

ASJC Scopus subject areas

  • Genetics

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