MULTIPRINS: a method for multicolour primed in situ labelling

E. V. Volpi; A. Baldini

doi:10.1007/BF00710131

MULTIPRINS: a method for multicolour primed in situ labelling

E. V. Volpi, A. Baldini

www.neuromed.it

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Abstract

With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

Original language	English
Pages (from-to)	257-260
Number of pages	4
Journal	Chromosome Research
Volume	1
Issue number	4
DOIs	https://doi.org/10.1007/BF00710131
Publication status	Published - Nov 1993

Keywords

fluorescence labelling
MULTIPRINS
repetitive DNA mapping

ASJC Scopus subject areas

Genetics

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10.1007/BF00710131

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abstract = "With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.",

keywords = "fluorescence labelling, MULTIPRINS, repetitive DNA mapping",

author = "Volpi, {E. V.} and A. Baldini",

year = "1993",

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AU - Baldini, A.

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N2 - With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

AB - With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

KW - fluorescence labelling

KW - MULTIPRINS

KW - repetitive DNA mapping

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MULTIPRINS: a method for multicolour primed in situ labelling

Abstract

Keywords

ASJC Scopus subject areas

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