Multiscale characterization of a chimeric biomimetic polypeptide for stem cell culture

F. Sbrana, C. Fotia, A. Bracalello, N. Baldini, G. Marletta, G. Ciapetti, B. Bochicchio, M. Vassalli

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mesenchymal stem cells have attracted great interest in the field of tissue engineering and regenerative medicine because of their multipotentiality and relative ease of isolation from adult tissues. The medical application of this cellular system requires the inclusion in a growth and delivery scaffold that is crucial for the clinical effectiveness of the therapy. In particular, the ideal scaffolding material should have the needed porosity and mechanical strength to allow a good integration with the surrounding tissues, but it should also assure high biocompatibility and full resorbability. For such a purpose, protein-inspired biomaterials and, in particular, elastomeric-derived polypeptides are playing a major role, in which they are expected to fulfil many of the biological and mechanical requirements. A specific chimeric protein, designed starting from elastin, resilin and collagen sequences, was characterized over different length scales. Single-molecule mechanics, aggregation properties and compatibility with human mesenchymal stem cells were tested, showing that the engineered compound is a good candidate as a stem cell scaffold to be used in tissue engineering applications.

Original languageEnglish
Article number046007
JournalBioinspiration and Biomimetics
Volume7
Issue number4
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Biomimetics
Polypeptides
Tissue Engineering
Stem cells
Mesenchymal Stromal Cells
Cell culture
Stem Cells
Cell Culture Techniques
Tissue engineering
Peptides
Regenerative Medicine
Elastin
Porosity
Biocompatible Materials
Mechanics
Tissue
Proteins
Collagen
Medical applications
Scaffolds (biology)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Molecular Medicine
  • Medicine(all)

Cite this

Multiscale characterization of a chimeric biomimetic polypeptide for stem cell culture. / Sbrana, F.; Fotia, C.; Bracalello, A.; Baldini, N.; Marletta, G.; Ciapetti, G.; Bochicchio, B.; Vassalli, M.

In: Bioinspiration and Biomimetics, Vol. 7, No. 4, 046007, 12.2012.

Research output: Contribution to journalArticle

Sbrana, F. ; Fotia, C. ; Bracalello, A. ; Baldini, N. ; Marletta, G. ; Ciapetti, G. ; Bochicchio, B. ; Vassalli, M. / Multiscale characterization of a chimeric biomimetic polypeptide for stem cell culture. In: Bioinspiration and Biomimetics. 2012 ; Vol. 7, No. 4.
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