Murine leukemia virus: detection of unintegrated double stranded DNA forms of the provirus

A. M. Gianni; D. Smotkin; R. A. Weinberg

Murine leukemia virus: detection of unintegrated double stranded DNA forms of the provirus

A. M. Gianni, D. Smotkin, R. A. Weinberg

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

Infection of JLS V9 (mouse bone marrow) cells with Moloney murine leukemia virus leads to appearance of virus specific DNA in the low molecular weight DNA fraction from infected cells. Most of this DNA is double stranded. Two discrete forms of double stranded DNA, both of molecular weight 6x10⁶, have been characterized. One of these forms is closed circular, supercoiled DNA; the other form is a mixture of nicked circlar and linear DNA.

Original language	English
Pages (from-to)	447-451
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	72
Issue number	2
Publication status	Published - 1975

ASJC Scopus subject areas

General
Genetics

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title = "Murine leukemia virus: detection of unintegrated double stranded DNA forms of the provirus",

abstract = "Infection of JLS V9 (mouse bone marrow) cells with Moloney murine leukemia virus leads to appearance of virus specific DNA in the low molecular weight DNA fraction from infected cells. Most of this DNA is double stranded. Two discrete forms of double stranded DNA, both of molecular weight 6x106, have been characterized. One of these forms is closed circular, supercoiled DNA; the other form is a mixture of nicked circlar and linear DNA.",

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AU - Weinberg, R. A.

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N2 - Infection of JLS V9 (mouse bone marrow) cells with Moloney murine leukemia virus leads to appearance of virus specific DNA in the low molecular weight DNA fraction from infected cells. Most of this DNA is double stranded. Two discrete forms of double stranded DNA, both of molecular weight 6x106, have been characterized. One of these forms is closed circular, supercoiled DNA; the other form is a mixture of nicked circlar and linear DNA.

AB - Infection of JLS V9 (mouse bone marrow) cells with Moloney murine leukemia virus leads to appearance of virus specific DNA in the low molecular weight DNA fraction from infected cells. Most of this DNA is double stranded. Two discrete forms of double stranded DNA, both of molecular weight 6x106, have been characterized. One of these forms is closed circular, supercoiled DNA; the other form is a mixture of nicked circlar and linear DNA.

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