Murine monoclonal antibody recognizing a 90-kDa cell surface determinant selectively lost by multi-drug-resistant variants of CEM cells

M. Cianfriglia, C. Cenciarelli, M. Tombesi, S. Barca, M. Mariani, S. Morrone, A. Santoni, P. Samoggia, M. Alessio, F. Malavasi

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a murine IgG1 monoclonal antibody (MAb56), specific for a cell-surface protein structure (MC56 determinant) expressed by the human CEM cell line. A large band of approximately 90 kDa was identified as the main specific component of the MC56 determinant. Such a 90-kDa protein is significantly associated with the drug-sensitive phenotype, its expression being progressively reduced quantitatively in multi-drug-resistant (MDR) variants of CEM cells, according to the extent of drug resistance. In addition, the MC56 determinant is expressed de novo in drug-sensitive revertant cell lines derived from MDR cells and unreactive with the MAb56. The MAb56 shows a high affinity towards the immunizing drug-sensitive CEM cell line (Ka = 1.86 x 109 L/mole) while not binding to MDR cell variants. The expression of the MC56 molecule on a variety of human cells and tissues makes such a cellular determinant a candidate as a marker for studying the MDR phenomenon both in vivo and in vitro.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalInternational Journal of Cancer
Volume45
Issue number1
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint

Dive into the research topics of 'Murine monoclonal antibody recognizing a 90-kDa cell surface determinant selectively lost by multi-drug-resistant variants of CEM cells'. Together they form a unique fingerprint.

Cite this