Murine peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived NK cells

Nissim Pinhas, Michal Sternberg-Simon, Laura Chiossone, Gitit Lavy-Shahaf, Thierry Walzer, Eric Vivier, Ramit Mehr

Research output: Contribution to journalArticle

Abstract

Murine NK cells can be divided by the expression of two cell surface markers, CD27 and Mac-1 (a.k.a. CD11b), into four separate subsets. These subsets suggest a linear development model: CD27- Mac-1- → CD27+ Mac-1- → CD27+ Mac-1+ → CD27- Mac-1+. Here, we used a combination of BrdU labeling experiments and mathematical modeling to gain insights regarding NK-cell development in mouse bone marrow (BM), spleen and liver. The modeling results that best fit the experimental data show that the majority of NK cells already express CD27 upon entering the NK-cell developmental pathway. Additionally, only a small fraction of NK cells exit the BM to other sites, suggesting that peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived mature NK cells.

Original languageEnglish
Pages (from-to)1258-1270
Number of pages13
JournalEuropean Journal of Immunology
Volume46
Issue number5
DOIs
Publication statusPublished - May 1 2016

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Keywords

  • CD11b
  • CD27
  • Computer simulation
  • Mathematical model
  • Natural killer cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Pinhas, N., Sternberg-Simon, M., Chiossone, L., Lavy-Shahaf, G., Walzer, T., Vivier, E., & Mehr, R. (2016). Murine peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived NK cells. European Journal of Immunology, 46(5), 1258-1270. https://doi.org/10.1002/eji.201545847