Muscle-derived hematopoietic stem cells are hematopoietic in origin

Shannon L. McKinney-Freeman, Kathyjo A. Jackson, Fernando D. Camargo, Giuliana Ferrari, Fulvio Mavilio, Margaret A. Goodell

Research output: Contribution to journalArticlepeer-review

Abstract

It has recently been shown that mononuclear cells from murine skeletal muscle contain the potential to repopulate all major peripheral blood lineages in lethally irradiated mice, but the origin of this activity is unknown. We have fractionated muscle cells on the basis of hematopoietic markers to show that the active population exclusively expresses the hematopoietic stem cell antigens Sca-1 and CD45. Muscle cells obtained from 6- to 8-week-old C57BL/6-CD45.1 mice and enriched for cells expressing Sca-1 and CD45 were able to generate hematopoietic but not myogenic colonies in vitro and repopulated multiple hematopoietic lineages of lethally irradiated C57BL/6-CD45.2 mice. These data show that muscle-derived hematopoietic stem cells are likely derived from the hematopoietic system and are a result not of transdifferentiation of myogenic stem cells but instead of the presence of substantial numbers of hematopoietic stem cells in the muscle. Although CD45-negative cells were highly myogenic in vitro and in vivo, CD45-positive muscle-derived cells displayed only very limited myogenic activity and only in vivo.

Original languageEnglish
Pages (from-to)1341-1346
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number3
DOIs
Publication statusPublished - Feb 5 2002

ASJC Scopus subject areas

  • General
  • Genetics

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