Abstract
A high level of glucose-6-phosphate dehydrogenase (G6PD) activity was observed in myoblasts and myotubes from normal human and mouse cell cultures. However, only a residual amount of activity was observed in myoblasts and myotubes obtained from G6PD-deficient patients (G6PD Mediterranean). Hybrids were formed by the fusion of normal (from human and mouse) and G6PD-deficient myoblasts (from the patients). These hybrids contained a high level of G6PD activity. Hoechst staining permitted to confirm that the enzymatic activity was not restrained to a domain near the competent nuclei. These results suggest that myoblast transplantation could be used to restore normal enzymatic activity in metabolic myopathies.
Original language | English |
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Pages (from-to) | 594-600 |
Number of pages | 7 |
Journal | Muscle and Nerve |
Volume | 16 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1993 |
Keywords
- fluorescent latex microspheres
- glucose-6-phosphate dehydrogenase
- hybrid myotubes
- muscle cell culture
- myoblast transplantation
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)