MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

A. I. Tsonis, P. Zisimopoulou, K. Lazaridis, J. Tzartos, E. Matsigkou, V. Zouvelou, R. Mantegazza, C. Antozzi, F. Andreetta, A. Evoli, F. Deymeer, G. Saruhan-Direskeneli, H. Durmus, T. Brenner, A. Vaknin, S. Berrih-Aknin, A. Behin, T. Sharshar, M. De Baets, M. LosenP. Martinez-Martinez, K. A. Kleopa, E. Zamba-Papanicolaou, T. Kyriakides, A. Kostera-Pruszczyk, P. Szczudlik, B. Szyluk, D. Lavrnic, I. Basta, S. Peric, C. Tallaksen, A. Maniaol, C. Casasnovas Pons, J. Pitha, M. Jakubíkova, F. Hanisch, S. J. Tzartos

Research output: Contribution to journalArticle

Abstract

Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

Original languageEnglish
Pages (from-to)10-17
Number of pages8
JournalJournal of Neuroimmunology
Volume284
DOIs
Publication statusPublished - Jul 15 2015

Fingerprint

Myasthenia Gravis
Autoantibodies
Phosphotransferases
Muscles
Antibodies
Thymus Hyperplasia
Neuromyelitis Optica
Multiple Sclerosis
Radioimmunoassay
Serum

Keywords

  • Autoantibodies
  • Cell based assay
  • Diagnosis
  • MuSK
  • Myasthenia gravis

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology
  • Medicine(all)

Cite this

Tsonis, A. I., Zisimopoulou, P., Lazaridis, K., Tzartos, J., Matsigkou, E., Zouvelou, V., ... Tzartos, S. J. (2015). MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study. Journal of Neuroimmunology, 284, 10-17. https://doi.org/10.1016/j.jneuroim.2015.04.015

MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study. / Tsonis, A. I.; Zisimopoulou, P.; Lazaridis, K.; Tzartos, J.; Matsigkou, E.; Zouvelou, V.; Mantegazza, R.; Antozzi, C.; Andreetta, F.; Evoli, A.; Deymeer, F.; Saruhan-Direskeneli, G.; Durmus, H.; Brenner, T.; Vaknin, A.; Berrih-Aknin, S.; Behin, A.; Sharshar, T.; De Baets, M.; Losen, M.; Martinez-Martinez, P.; Kleopa, K. A.; Zamba-Papanicolaou, E.; Kyriakides, T.; Kostera-Pruszczyk, A.; Szczudlik, P.; Szyluk, B.; Lavrnic, D.; Basta, I.; Peric, S.; Tallaksen, C.; Maniaol, A.; Casasnovas Pons, C.; Pitha, J.; Jakubíkova, M.; Hanisch, F.; Tzartos, S. J.

In: Journal of Neuroimmunology, Vol. 284, 15.07.2015, p. 10-17.

Research output: Contribution to journalArticle

Tsonis, AI, Zisimopoulou, P, Lazaridis, K, Tzartos, J, Matsigkou, E, Zouvelou, V, Mantegazza, R, Antozzi, C, Andreetta, F, Evoli, A, Deymeer, F, Saruhan-Direskeneli, G, Durmus, H, Brenner, T, Vaknin, A, Berrih-Aknin, S, Behin, A, Sharshar, T, De Baets, M, Losen, M, Martinez-Martinez, P, Kleopa, KA, Zamba-Papanicolaou, E, Kyriakides, T, Kostera-Pruszczyk, A, Szczudlik, P, Szyluk, B, Lavrnic, D, Basta, I, Peric, S, Tallaksen, C, Maniaol, A, Casasnovas Pons, C, Pitha, J, Jakubíkova, M, Hanisch, F & Tzartos, SJ 2015, 'MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study', Journal of Neuroimmunology, vol. 284, pp. 10-17. https://doi.org/10.1016/j.jneuroim.2015.04.015
Tsonis, A. I. ; Zisimopoulou, P. ; Lazaridis, K. ; Tzartos, J. ; Matsigkou, E. ; Zouvelou, V. ; Mantegazza, R. ; Antozzi, C. ; Andreetta, F. ; Evoli, A. ; Deymeer, F. ; Saruhan-Direskeneli, G. ; Durmus, H. ; Brenner, T. ; Vaknin, A. ; Berrih-Aknin, S. ; Behin, A. ; Sharshar, T. ; De Baets, M. ; Losen, M. ; Martinez-Martinez, P. ; Kleopa, K. A. ; Zamba-Papanicolaou, E. ; Kyriakides, T. ; Kostera-Pruszczyk, A. ; Szczudlik, P. ; Szyluk, B. ; Lavrnic, D. ; Basta, I. ; Peric, S. ; Tallaksen, C. ; Maniaol, A. ; Casasnovas Pons, C. ; Pitha, J. ; Jakubíkova, M. ; Hanisch, F. ; Tzartos, S. J. / MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study. In: Journal of Neuroimmunology. 2015 ; Vol. 284. pp. 10-17.
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AU - Tsonis, A. I.

AU - Zisimopoulou, P.

AU - Lazaridis, K.

AU - Tzartos, J.

AU - Matsigkou, E.

AU - Zouvelou, V.

AU - Mantegazza, R.

AU - Antozzi, C.

AU - Andreetta, F.

AU - Evoli, A.

AU - Deymeer, F.

AU - Saruhan-Direskeneli, G.

AU - Durmus, H.

AU - Brenner, T.

AU - Vaknin, A.

AU - Berrih-Aknin, S.

AU - Behin, A.

AU - Sharshar, T.

AU - De Baets, M.

AU - Losen, M.

AU - Martinez-Martinez, P.

AU - Kleopa, K. A.

AU - Zamba-Papanicolaou, E.

AU - Kyriakides, T.

AU - Kostera-Pruszczyk, A.

AU - Szczudlik, P.

AU - Szyluk, B.

AU - Lavrnic, D.

AU - Basta, I.

AU - Peric, S.

AU - Tallaksen, C.

AU - Maniaol, A.

AU - Casasnovas Pons, C.

AU - Pitha, J.

AU - Jakubíkova, M.

AU - Hanisch, F.

AU - Tzartos, S. J.

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

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KW - Cell based assay

KW - Diagnosis

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