Mussel micronucleus cytome assay.

Claudia Bolognesi, Michael Fenech

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The micronucleus (MN) assay is one of the most widely used genotoxicity biomarkers in aquatic organisms, providing an efficient measure of chromosomal DNA damage occurring as a result of either chromosome breakage or chromosome mis-segregation during mitosis. The MN assay is today applied in laboratory and field studies using hemocytes and gill cells from bivalves, mainly from the genera Mytilus. These represent 'sentinel' organisms because of their ability to survive under polluted conditions and to accumulate both organic and inorganic pollutants. Because the mussel MN assay also includes scoring of different cell types, including necrotic and apoptotic cells and other nuclear anomalies, it is in effect an MN cytome assay. The mussel MN cytome (MUMNcyt) assay protocol we describe here reports the recommended experimental design, sample size, cell preparation, cell fixation and staining methods. The protocol also includes criteria and photomicrographs for identifying different cell types and scoring criteria for micronuclei (MNi) and nuclear buds. The complete procedure requires approximately 10 h for each experimental point/sampling station (ten animals).

Original languageEnglish
Pages (from-to)1125-1137
Number of pages13
JournalNature Protocols
Volume7
Issue number6
DOIs
Publication statusPublished - 2012

Fingerprint

Micronucleus Tests
Bivalvia
Assays
Chromosomes
Aquatic organisms
Mytilus
Biomarkers
Chromosome Breakage
Hemocytes
Aquatic Organisms
Chromosome Segregation
Design of experiments
Mitosis
Animals
Sample Size
DNA Damage
Cells
Sampling
Research Design
Staining and Labeling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mussel micronucleus cytome assay. / Bolognesi, Claudia; Fenech, Michael.

In: Nature Protocols, Vol. 7, No. 6, 2012, p. 1125-1137.

Research output: Contribution to journalArticle

Bolognesi, Claudia ; Fenech, Michael. / Mussel micronucleus cytome assay. In: Nature Protocols. 2012 ; Vol. 7, No. 6. pp. 1125-1137.
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