Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of humans exposed to therapeutical coal tar

E. Clonfero, M. Zordan, D. Cottica, P. Venier, L. Pozzoli, E. L. Cardin, F. Sarto, A. G. Levis

Research output: Contribution to journalArticle

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Abstract

The urinary mutagenicity and the excretion of polycyclic aromatic hydrocarbons (PAH) in three non-smoking male patients, treated for psoriasis with cutaneous applications of crude coal tar, were analysed. Mutagenicity of the urinary extracts was measured by the plate incorporation assay using Salmonella typhimurium strains TA 98 and TA 100 in the presence of liver S9 fraction from Aroclor-induced rats with or without β-glucuronidase. After concentration, hydrolysis and reduction of the urine sample, PAH levels were measured by high resolution gas chromatography/mass spectrometry. Following cutaneous treatment with coal tar, the urine of all three subjects showed noticeable levels of PAH and/or metabolites and marked mutagenicity both on strain TA 98 and TA 100 in the presence of S9 fraction. The addition of β-glucuronidase increased the mutagenicity of the urinary extracts, the maximum values being attained on strain TA 100 in the presence of both microsomal fraction and deconjugating enzymes. The mutagenicity of urinary extracts from subjects treated therapeutically with crude coal tar was correlated (r = 0.788, P <0.01) with the total PAH levels in their urine. The PAH excreted in urine were mainly low molecular weight compounds, while benzo[a]anthracene was present in scarce amounts and the excretion of benzo[a]pyrene did not increase following the cutaneous exposure to the crude coal tar.

Original languageEnglish
Pages (from-to)819-823
Number of pages5
JournalCarcinogenesis
Volume7
Issue number5
DOIs
Publication statusPublished - May 1986

Fingerprint

Coal Tar
Coal tar
Polycyclic Aromatic Hydrocarbons
Hydrocarbons
Polycyclic aromatic hydrocarbons
Urine
Glucuronidase
Skin
Aroclors
Gas Chromatography
Salmonella
Anthracene
Benzo(a)pyrene
Pyrene
Mass Spectrometry
Salmonella typhimurium
Metabolites
Psoriasis
Gas chromatography
Liver

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Clonfero, E., Zordan, M., Cottica, D., Venier, P., Pozzoli, L., Cardin, E. L., ... Levis, A. G. (1986). Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of humans exposed to therapeutical coal tar. Carcinogenesis, 7(5), 819-823. https://doi.org/10.1093/carcin/7.5.819

Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of humans exposed to therapeutical coal tar. / Clonfero, E.; Zordan, M.; Cottica, D.; Venier, P.; Pozzoli, L.; Cardin, E. L.; Sarto, F.; Levis, A. G.

In: Carcinogenesis, Vol. 7, No. 5, 05.1986, p. 819-823.

Research output: Contribution to journalArticle

Clonfero, E, Zordan, M, Cottica, D, Venier, P, Pozzoli, L, Cardin, EL, Sarto, F & Levis, AG 1986, 'Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of humans exposed to therapeutical coal tar', Carcinogenesis, vol. 7, no. 5, pp. 819-823. https://doi.org/10.1093/carcin/7.5.819
Clonfero, E. ; Zordan, M. ; Cottica, D. ; Venier, P. ; Pozzoli, L. ; Cardin, E. L. ; Sarto, F. ; Levis, A. G. / Mutagenic activity and polycyclic aromatic hydrocarbon levels in urine of humans exposed to therapeutical coal tar. In: Carcinogenesis. 1986 ; Vol. 7, No. 5. pp. 819-823.
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