Mutagenic relevance of rat hepatocyte nuclei in the activation and inactivation of xenobiotica cyclophosphamide and epichlorohydrin activity on the yeasts S. pombe and S. cerevisiae

L. Migliore, A. M. Rossi, N. Loprieno, M. Romano, M. Salmona

Research output: Contribution to journalArticlepeer-review

Abstract

The influence on the mutagenicity of cyclophosphamide (Cy) and epichlorohydrin (ECH), of liver nuclei and hepatic post-mitochondrial (S9) preparations from phenobarbital-treated rats, was examined. The study was conducted in vitro, with 2 yeasts, Schisosaccharomyces pombe (P1 strain) which allows the detection of forward mutations, and Saccharomyces cerevisiae (D5 strain), in which the induction of different genetic effects, such as mitotic recombination, can be evaluated. The results indicated that the nuclear fraction has a qualitative capacity for biotransformation of compounds, overlapping that of S9. From a quantitative point of view, the Cy-activating capacity of the nuclear fraction was twice as high as that of S9 whereas the two fractions showed a similar ECH-inactivating ability. The present study strengthens the hypothesis of the relevance of nuclei as a site of metabolic activation and inactivation of exogenous compounds.

Original languageEnglish
Pages (from-to)313-323
Number of pages11
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume111
Issue number3
DOIs
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Fingerprint

Dive into the research topics of 'Mutagenic relevance of rat hepatocyte nuclei in the activation and inactivation of xenobiotica cyclophosphamide and epichlorohydrin activity on the yeasts S. pombe and S. cerevisiae'. Together they form a unique fingerprint.

Cite this