Mutagenicity of polycyclic aromatic hydrocarbon fractions extracted from urban air particulates

S. De Flora, M. Bagnasco, A. Izzotti, F. D'Agostini, M. Pala, F. Valerio

Research output: Contribution to journalArticle

Abstract

Polycyclic aromatic hydrocarbon (PAH) fractions, purified from extracts of airborne particles collected in the area of Genoa municipality, were assayed for mutagenicity in the Salmonella/microsome test. PAH fractions accounted for only a portion of the total mutagenic activity and also displayed a different specificity of genetic activity, as compared to unfractionated material. The analysis of 224 samples collected from January 1986 to November 1987 in 10 different localities led to a large number of positive results in strain TA100 with S9 mix and, less frequently, also in TA98 without metabolic activation. Mutagenicity was related to the intensity of anthropogenic atmospheric pollution, and showed some seasonal variations, although it was not possible to discriminate particular sources of pollution on the basis of mutagenicity patterns. The mutagenic potency in TA100 (S9+) of airborne PAH fractions was significantly correlated with the concentration of individual PAHs in most of the monitored localities. The spectrum of mutagenicity of monthly samples pooled from several localities in S. typhimurium strains, with and without S9 mix, provided evidence for some contribution of nitro derivatives of PAHs or possibly also of other compounds present in the same fractions. The results obtained are discussed in view of their predictive value as indicators of potential health hazards, and of the reliability of this biological tool as a complement to chemical analyses in the evaluation of ambient air pollution.

Original languageEnglish
Pages (from-to)305-318
Number of pages14
JournalMutation Research/Genetic Toxicology
Volume224
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Polycyclic Aromatic Hydrocarbons
Air
Polycyclic aromatic hydrocarbons
Pollution
Health hazards
Salmonella
Air Pollution
Microsomes
Air pollution
Chemical activation
Derivatives
Health

Keywords

  • Airborne particulates
  • Atmospheric pollution
  • Polycyclic aromatic hydrocarbons
  • Salmonella mutagenicity assay

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

Mutagenicity of polycyclic aromatic hydrocarbon fractions extracted from urban air particulates. / De Flora, S.; Bagnasco, M.; Izzotti, A.; D'Agostini, F.; Pala, M.; Valerio, F.

In: Mutation Research/Genetic Toxicology, Vol. 224, No. 2, 1989, p. 305-318.

Research output: Contribution to journalArticle

De Flora, S. ; Bagnasco, M. ; Izzotti, A. ; D'Agostini, F. ; Pala, M. ; Valerio, F. / Mutagenicity of polycyclic aromatic hydrocarbon fractions extracted from urban air particulates. In: Mutation Research/Genetic Toxicology. 1989 ; Vol. 224, No. 2. pp. 305-318.
@article{51c5ae00913045e6930bb330afa6d60d,
title = "Mutagenicity of polycyclic aromatic hydrocarbon fractions extracted from urban air particulates",
abstract = "Polycyclic aromatic hydrocarbon (PAH) fractions, purified from extracts of airborne particles collected in the area of Genoa municipality, were assayed for mutagenicity in the Salmonella/microsome test. PAH fractions accounted for only a portion of the total mutagenic activity and also displayed a different specificity of genetic activity, as compared to unfractionated material. The analysis of 224 samples collected from January 1986 to November 1987 in 10 different localities led to a large number of positive results in strain TA100 with S9 mix and, less frequently, also in TA98 without metabolic activation. Mutagenicity was related to the intensity of anthropogenic atmospheric pollution, and showed some seasonal variations, although it was not possible to discriminate particular sources of pollution on the basis of mutagenicity patterns. The mutagenic potency in TA100 (S9+) of airborne PAH fractions was significantly correlated with the concentration of individual PAHs in most of the monitored localities. The spectrum of mutagenicity of monthly samples pooled from several localities in S. typhimurium strains, with and without S9 mix, provided evidence for some contribution of nitro derivatives of PAHs or possibly also of other compounds present in the same fractions. The results obtained are discussed in view of their predictive value as indicators of potential health hazards, and of the reliability of this biological tool as a complement to chemical analyses in the evaluation of ambient air pollution.",
keywords = "Airborne particulates, Atmospheric pollution, Polycyclic aromatic hydrocarbons, Salmonella mutagenicity assay",
author = "{De Flora}, S. and M. Bagnasco and A. Izzotti and F. D'Agostini and M. Pala and F. Valerio",
year = "1989",
doi = "10.1016/0165-1218(89)90169-9",
language = "English",
volume = "224",
pages = "305--318",
journal = "Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure",
issn = "0165-1218",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Mutagenicity of polycyclic aromatic hydrocarbon fractions extracted from urban air particulates

AU - De Flora, S.

AU - Bagnasco, M.

AU - Izzotti, A.

AU - D'Agostini, F.

AU - Pala, M.

AU - Valerio, F.

PY - 1989

Y1 - 1989

N2 - Polycyclic aromatic hydrocarbon (PAH) fractions, purified from extracts of airborne particles collected in the area of Genoa municipality, were assayed for mutagenicity in the Salmonella/microsome test. PAH fractions accounted for only a portion of the total mutagenic activity and also displayed a different specificity of genetic activity, as compared to unfractionated material. The analysis of 224 samples collected from January 1986 to November 1987 in 10 different localities led to a large number of positive results in strain TA100 with S9 mix and, less frequently, also in TA98 without metabolic activation. Mutagenicity was related to the intensity of anthropogenic atmospheric pollution, and showed some seasonal variations, although it was not possible to discriminate particular sources of pollution on the basis of mutagenicity patterns. The mutagenic potency in TA100 (S9+) of airborne PAH fractions was significantly correlated with the concentration of individual PAHs in most of the monitored localities. The spectrum of mutagenicity of monthly samples pooled from several localities in S. typhimurium strains, with and without S9 mix, provided evidence for some contribution of nitro derivatives of PAHs or possibly also of other compounds present in the same fractions. The results obtained are discussed in view of their predictive value as indicators of potential health hazards, and of the reliability of this biological tool as a complement to chemical analyses in the evaluation of ambient air pollution.

AB - Polycyclic aromatic hydrocarbon (PAH) fractions, purified from extracts of airborne particles collected in the area of Genoa municipality, were assayed for mutagenicity in the Salmonella/microsome test. PAH fractions accounted for only a portion of the total mutagenic activity and also displayed a different specificity of genetic activity, as compared to unfractionated material. The analysis of 224 samples collected from January 1986 to November 1987 in 10 different localities led to a large number of positive results in strain TA100 with S9 mix and, less frequently, also in TA98 without metabolic activation. Mutagenicity was related to the intensity of anthropogenic atmospheric pollution, and showed some seasonal variations, although it was not possible to discriminate particular sources of pollution on the basis of mutagenicity patterns. The mutagenic potency in TA100 (S9+) of airborne PAH fractions was significantly correlated with the concentration of individual PAHs in most of the monitored localities. The spectrum of mutagenicity of monthly samples pooled from several localities in S. typhimurium strains, with and without S9 mix, provided evidence for some contribution of nitro derivatives of PAHs or possibly also of other compounds present in the same fractions. The results obtained are discussed in view of their predictive value as indicators of potential health hazards, and of the reliability of this biological tool as a complement to chemical analyses in the evaluation of ambient air pollution.

KW - Airborne particulates

KW - Atmospheric pollution

KW - Polycyclic aromatic hydrocarbons

KW - Salmonella mutagenicity assay

UR - http://www.scopus.com/inward/record.url?scp=0024434479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024434479&partnerID=8YFLogxK

U2 - 10.1016/0165-1218(89)90169-9

DO - 10.1016/0165-1218(89)90169-9

M3 - Article

C2 - 2677712

AN - SCOPUS:0024434479

VL - 224

SP - 305

EP - 318

JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

SN - 0165-1218

IS - 2

ER -