Mutation analysis of KRAS in primary colorectal cancer and matched metastases by means of highly sensitivity molecular assay

Umberto Miglio, Rosanna Mezzapelle, Alessia Paganotti, Sara Allegrini, Claudia Veggiani, Jlenia Antona, Sergio Gentilli, Guido Monga, Oscar Alabiso, Renzo Boldorini

Research output: Contribution to journalArticle

Abstract

Mutation analysis of KRAS is needed before starting treatment with anti-EGFR monoclonal antibodies in patients with metastatic colorectal cancer (CRC). In most of the cases, testing is performed on primary tumors, assuming that KRAS mutation status does not change in metastasis although correlation studies gave conflicting results. We evaluated the KRAS status concordance rate between primary tumors and related metastasis using a highly sensitive molecular assay. Forty-five primary tumors and related metastases from patients with CRC (28/45 male-62.2% and 17/45 female-37.8%; mean age 66.4 years) were analyzed by using TheraScreen: KRAS mutational kit. Metastatic samples were collected from lymph nodes (8/45-17.8%) and visceral sites (37/45-82.2%); 23 were synchronous (49%) and 22 were metachronous (51%), obtained after a mean of 30.8 months after the first diagnosis of CRC. Twenty-eight patients had KRAS mutations in both primary CRC and related metastases (62.2%). No differences in type and frequency of mutations were identified, despite different metastatic sites and time of onset of metastatic disease. Our results indicate that the mutation status of KRAS is the same in primary CRC and metastasis, suggesting that in clinical practice, KRAS testing can be performed on both tumor tissues when using a highly sensitive molecular assay.

Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalPathology Research and Practice
Volume209
Issue number4
DOIs
Publication statusPublished - Apr 2013

Keywords

  • Colorectal cancer
  • KRAS gene
  • Mutational analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

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    Miglio, U., Mezzapelle, R., Paganotti, A., Allegrini, S., Veggiani, C., Antona, J., Gentilli, S., Monga, G., Alabiso, O., & Boldorini, R. (2013). Mutation analysis of KRAS in primary colorectal cancer and matched metastases by means of highly sensitivity molecular assay. Pathology Research and Practice, 209(4), 233-236. https://doi.org/10.1016/j.prp.2013.02.006