Mutations at coding mononucleotide repeats in gastric cancer with the microsatellite mutator phenotype

Laura Ottini, Mario Falchetti, Cristina D'Amico, Andrea Amorosi, Calogero Saieva, Giovanna Masala, Luigi Frati, Alessandro Cama, Domenico Palli, Renato Mariani-Costantini

Research output: Contribution to journalArticlepeer-review

Abstract

We analysed 50 gastric carcinomas (GCs) to verify whether mutations at coding repeats were associated with microsatellite instability (MSI). The tumors included: ten cases with no MSI, 14 cases with MSI = 1 locus, 13 cases with MSI = two loci and 13 cases with MS ≤ 3 loci. We investigated coding repeats within the TGF-β RII, IGFIIR, BAX, hMSH6, hMSH3 and BRCA2 genes, The TGF-β RII, IGFIIR, BAX, hMSH6 and hMSH3 repeats were altered in 11 (22%), five (10%), four (8%), 16 (32%) and five (10%) cases respectively. Mutations occurred only in MSI-positive (MSI+) tumors and correlated with increasing MSI levels. No alterations of the BRCA2 repeat were found. Mutations in genes other than hMSH6 were strongly associated to hMSH6 mutations, suggesting a key role of this gene. The non-coding BAT-26 and E-Cadherin in 3' UTR poly(A)8/(T)15 repeats were analysed in 44 of the 50 cases. Novel tumor-associated alleles were observed only in MSI-positive GCs and were in most cases associated with mutations at coding repeats. Further investigations with BAT-40 confirmed that four cases manifested mononucleotide repeat alterations restricted to hMSH6 and one case to TGF-β RII. A subset of tumors with MSI at two or more dinucleotide loci resulted negative for mutations at coding and non-coding mononucleotide repeats.

Original languageEnglish
Pages (from-to)2767-2772
Number of pages6
JournalOncogene
Volume16
Issue number21
Publication statusPublished - May 28 1998

Keywords

  • BAX
  • BRCA2
  • Gastric cancer
  • hMSH3
  • hMSH6
  • IGFIIR
  • Microsatellite instability
  • Mutation
  • TGFβ-RII

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Fingerprint Dive into the research topics of 'Mutations at coding mononucleotide repeats in gastric cancer with the microsatellite mutator phenotype'. Together they form a unique fingerprint.

Cite this