Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability

Giuseppe Giannini, Christian Rinaldi, Elisabetta Ristori, Maria Irene Ambrosini, Fabio Cerignoli, Alessandra Viel, Ettore Bidoli, Silvia Berni, Giulia D'Amati, Giovanni Scambia, Luigi Frati, Isabella Screpanti, Alberto Gulino

Research output: Contribution to journalArticle

Abstract

Frequent mutations of coding nucleotide repeats are thought to contribute significantly to carcinogenesis associated with microsatellite instability (MSI). We have shown that shortening of the poly(T)11 within the polypyrimidine stretch/accessory splicing signal of human MRE11 leads to the reduced expression and functional impairment of the MRE11/NBS1/RAD50 complex. This mutation was selectively found in mismatch repair (MMR) defective cell lines and potentially identifies MRE11 as a novel target for MSI. Here, we examined 70 microsatellite unstable primary human cancers and we report that MRE11 mutations occur in 83.7 and 50% of the colorectal and endometrial cancers, respectively. In the colorectal cancer series, mutated MRE11 is more frequently associated with advanced age at diagnosis and A/B stages. Biallelic mutations were present in 38.8% of the cases and more frequently associated with lower (G1/G2) grade tumors. Impaired MRE11 expression was prevalent in primary colorectal tumors with larger and biallelic shortening of the poly(T)11. Immunohistochemistry confirmed the impaired MRE11 expression and revealed NBS1-defective expression in MRE11 mutated cancers. Together with the observation that perturbation of the MRE11/NBS1/RAD50 complex predisposes to cancer, our work highlights MRE11 as a new common target in the MMR deficient tumorigenesis and suggests its role in colorectal carcinogenesis.

Original languageEnglish
Pages (from-to)2640-2647
Number of pages8
JournalOncogene
Volume23
Issue number15
DOIs
Publication statusPublished - Apr 8 2004

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Keywords

  • Colorectal cancer
  • DNA double strand breaks repair
  • MMR defect
  • MRE11
  • NBS1

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Giannini, G., Rinaldi, C., Ristori, E., Ambrosini, M. I., Cerignoli, F., Viel, A., Bidoli, E., Berni, S., D'Amati, G., Scambia, G., Frati, L., Screpanti, I., & Gulino, A. (2004). Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene, 23(15), 2640-2647. https://doi.org/10.1038/sj.onc.1207409