Mutations of the RAG3 gene encoding a regulator of fermentation in Kluyveromyces lactis are suppressed by a mutation of the transcription factor gene KlGCR1

Lorenza Tizzani, Micheline Wésolowski-Louvel, Valentina Forte, Federica Romitelli, Francesca Salani, Marc Lemaire, Helen Neil, Michele M. Bianchi

Research output: Contribution to journalArticle

Abstract

In Kluyveromyces lactis, Rag3 regulates both fermentative metabolism and thiamine biosynthesis. Regulation of fermentation is exerted at the level of transcription of KlPDC1. We have isolated and identified a mutation of the transcription factor KlGCR1, Klgcr1-1, which suppressed the fermentative- deficient phenotype associated with the RAG3 deletion. In the mutant, the transcription of KlPDC1 was restored. However, we found that the suppression was not specific to the RAG3 mutation, as the Klgcr1-1 mutation could also suppress the fermentative defect associated with mutation of Sck1, another regulator of glycolysis.

Original languageEnglish
Pages (from-to)675-682
Number of pages8
JournalFEMS Yeast Research
Volume7
Issue number5
DOIs
Publication statusPublished - Aug 2007

Keywords

  • Genetic suppressor
  • Pyruvate decarboxylase
  • Transcriptional regulation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

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