TY - JOUR
T1 - Mutations of the RAG3 gene encoding a regulator of fermentation in Kluyveromyces lactis are suppressed by a mutation of the transcription factor gene KlGCR1
AU - Tizzani, Lorenza
AU - Wésolowski-Louvel, Micheline
AU - Forte, Valentina
AU - Romitelli, Federica
AU - Salani, Francesca
AU - Lemaire, Marc
AU - Neil, Helen
AU - Bianchi, Michele M.
PY - 2007/8
Y1 - 2007/8
N2 - In Kluyveromyces lactis, Rag3 regulates both fermentative metabolism and thiamine biosynthesis. Regulation of fermentation is exerted at the level of transcription of KlPDC1. We have isolated and identified a mutation of the transcription factor KlGCR1, Klgcr1-1, which suppressed the fermentative- deficient phenotype associated with the RAG3 deletion. In the mutant, the transcription of KlPDC1 was restored. However, we found that the suppression was not specific to the RAG3 mutation, as the Klgcr1-1 mutation could also suppress the fermentative defect associated with mutation of Sck1, another regulator of glycolysis.
AB - In Kluyveromyces lactis, Rag3 regulates both fermentative metabolism and thiamine biosynthesis. Regulation of fermentation is exerted at the level of transcription of KlPDC1. We have isolated and identified a mutation of the transcription factor KlGCR1, Klgcr1-1, which suppressed the fermentative- deficient phenotype associated with the RAG3 deletion. In the mutant, the transcription of KlPDC1 was restored. However, we found that the suppression was not specific to the RAG3 mutation, as the Klgcr1-1 mutation could also suppress the fermentative defect associated with mutation of Sck1, another regulator of glycolysis.
KW - Genetic suppressor
KW - Pyruvate decarboxylase
KW - Transcriptional regulation
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U2 - 10.1111/j.1567-1364.2007.00219.x
DO - 10.1111/j.1567-1364.2007.00219.x
M3 - Article
C2 - 17559574
AN - SCOPUS:34447299978
VL - 7
SP - 675
EP - 682
JO - FEMS Yeast Research
JF - FEMS Yeast Research
SN - 1567-1356
IS - 5
ER -