Mutations spanning P53 exons 5-9 detected by non-isotopic RNAse cleavage assay and protein expression in human colon cancer

Stefania Tommasi, Marta Abatangelo, Rosanna Lacalamita, Severino Montemurro, Francesco Marzullo, Angelo Paradiso

Research output: Contribution to journalArticlepeer-review

Abstract

The non-isotopic assay (NIRCA), based on the observation that RNAse is able to specifically cleave a single mismatch in RNA/RNA duplexes, has been recently proposed to detect p53 mutations. To verify the use of this method as a valid screening for P53 mutations in a routinely collected cancer series, we used this assay on 3 cases with normal and 5 cases with abnormal P53 expression detected by Western blots. In all cases, P53 exons 5-6, 7 and 8-9 regions were analyzed. There were mutations only in the five overexpressed cases: two cases showed mutations in exon 5, one between intron 6 and exon 6 and two in the region spanning exons 8 and 9. Our experience showed NIRCA to be fast, reliable and providing the ability to study long target regions in a single step, thus making this assay useful for genetic screenings.

Original languageEnglish
Pages (from-to)40-42
Number of pages3
JournalCancer Genetics and Cytogenetics
Volume129
Issue number1
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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