Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release

Emilie Venereau, Maura Casalgrandi, Milena Schiraldi, Daniel J. Antoine, Angela Cattaneo, Francesco De Marchis, Jaron Liu, Antonella Antonelli, Alessandro Preti, Lorenzo Raeli, Sara Samadi Shams, Huan Yang, Luca Varani, Ulf Andersson, Kevin J. Tracey, Angela Bachi, Mariagrazia Uguccioni, Marco E. Bianchi

Research output: Contribution to journalArticle

338 Citations (Scopus)

Abstract

Tissue damage causes inflammation, by recruiting leukocytes and activating them to release proinflammatory mediators. We show that high-mobility group box 1 protein (HMGB1) orchestrates both processes by switching among mutually exclusive redox states. Reduced cysteines make HMGB1 a chemoattractant, whereas a disulfide bond makes it a proinflammatory cytokine and further cysteine oxidation to sulfonates by reactive oxygen species abrogates both activities. We show that leukocyte recruitment and activation can be separated. A nonoxidizable HMGB1 mutant in which serines replace all cysteines (3S-HMGB1) does not promote cytokine production, but is more effective than wild-type HMGB1 in recruiting leukocytes in vivo. BoxA, a HMGB1 inhibitor, interferes with leukocyte recruitment but not with activation. We detected the different redox forms of HMGB1 ex vivo within injured muscle. HMGB1 is completely reduced at first and disulfide-bonded later. Thus, HMGB1 orchestrates both key events in sterile inflammation, leukocyte recruitment and their induction to secrete inflammatory cytokines, by adopting mutually exclusive redox states.

Original languageEnglish
Pages (from-to)1519-1528
Number of pages10
JournalJournal of Experimental Medicine
Volume209
Issue number9
DOIs
Publication statusPublished - Aug 2012

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HMGB1 Protein
Oxidation-Reduction
Cytokines
Leukocytes
Cysteine
Disulfides
Inflammation
Chemotactic Factors
Serine
Reactive Oxygen Species
Muscles

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release. / Venereau, Emilie; Casalgrandi, Maura; Schiraldi, Milena; Antoine, Daniel J.; Cattaneo, Angela; De Marchis, Francesco; Liu, Jaron; Antonelli, Antonella; Preti, Alessandro; Raeli, Lorenzo; Shams, Sara Samadi; Yang, Huan; Varani, Luca; Andersson, Ulf; Tracey, Kevin J.; Bachi, Angela; Uguccioni, Mariagrazia; Bianchi, Marco E.

In: Journal of Experimental Medicine, Vol. 209, No. 9, 08.2012, p. 1519-1528.

Research output: Contribution to journalArticle

Venereau, E, Casalgrandi, M, Schiraldi, M, Antoine, DJ, Cattaneo, A, De Marchis, F, Liu, J, Antonelli, A, Preti, A, Raeli, L, Shams, SS, Yang, H, Varani, L, Andersson, U, Tracey, KJ, Bachi, A, Uguccioni, M & Bianchi, ME 2012, 'Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release', Journal of Experimental Medicine, vol. 209, no. 9, pp. 1519-1528. https://doi.org/10.1084/jem.20120189
Venereau, Emilie ; Casalgrandi, Maura ; Schiraldi, Milena ; Antoine, Daniel J. ; Cattaneo, Angela ; De Marchis, Francesco ; Liu, Jaron ; Antonelli, Antonella ; Preti, Alessandro ; Raeli, Lorenzo ; Shams, Sara Samadi ; Yang, Huan ; Varani, Luca ; Andersson, Ulf ; Tracey, Kevin J. ; Bachi, Angela ; Uguccioni, Mariagrazia ; Bianchi, Marco E. / Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release. In: Journal of Experimental Medicine. 2012 ; Vol. 209, No. 9. pp. 1519-1528.
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