MYC recruits the TIP60 histone acetyltransferase complex to chromatin

Scott R. Frank, Tiziana Parisi, Stefan Taubert, Paula Fernandez, Miriam Fuchs, Ho Man Chan, David M. Livingston, Bruno Amati

Research output: Contribution to journalArticlepeer-review

Abstract

The transcription factor MYC binds specific DNA sites in cellular chromatin and induces the acetylation of histones H3 and H4. However, the histone acetyltransferases (HATs) that are responsible for these modifications have not yet been identified. MYC associates with TRRAP, a subunit of distinct macromolecular complexes that contain the HATs GCN5/PCAF or TIP60. Although the association of MYC with GCN5 has been shown, its interaction with TIP60 has never been analysed. Here, we show that MYC associates with TIP60 and recruits it to chromatin in vivo with four other components of the TIP60 complex: TRRAP, p400, TIP48 and TIP49. Overexpression of enzymatically inactive TIP60 delays the MYC-induced acetylation of histone H4, and also reduces the level of MYC binding to chromatin. Thus, the TIP60 HAT complex is recruited to MYC-target genes and, probably with other other HATs, contributes to histone acetylation in response to mitogenic signals.

Original languageEnglish
Pages (from-to)575-580
Number of pages6
JournalEMBO Reports
Volume4
Issue number6
DOIs
Publication statusPublished - Jun 1 2003

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Molecular Biology
  • Biochemistry

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