Mycobacterium celeriflavum sp. Nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens

Abdolrazagh Hashemi Shahraki, Cengiz Çavuşoğlu, Emanuele Borroni, Parvin Heidarieh, Orhan Kaya Koksalan, Andrea Maurizio Cabibbe, Mohamad Hashemzadeh, Alessandro Mariottini, Ehsan Mostafavi, Davide Cittaro, Mohamad Mehdi Feizabadi, Dejan Lazarevic, Farhad Yaghmaei, Gian Lorenzo Molinari, Anna Camaggi, Enrico Tortoli

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0% 16S rRNA gene sequence similarity, and 91.5–96.5% similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9%. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207T (5DSM 46765T5JCM 18439T).

Original languageEnglish
Pages (from-to)510-515
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number2
DOIs
Publication statusPublished - 2015

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Mycobacterium
Mycobacterium moriokaense
Bacteria
bacterium
gene
bacteria
phylogenetics
Mycobacterium flavescens
Nucleotides
Mycolic Acids
Nontuberculous Mycobacteria
nucleotides
relatedness
targeting
Essential Genes
rRNA Genes
genome
phylogeny
testing
Genes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Mycobacterium celeriflavum sp. Nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens. / Shahraki, Abdolrazagh Hashemi; Çavuşoğlu, Cengiz; Borroni, Emanuele; Heidarieh, Parvin; Koksalan, Orhan Kaya; Cabibbe, Andrea Maurizio; Hashemzadeh, Mohamad; Mariottini, Alessandro; Mostafavi, Ehsan; Cittaro, Davide; Feizabadi, Mohamad Mehdi; Lazarevic, Dejan; Yaghmaei, Farhad; Molinari, Gian Lorenzo; Camaggi, Anna; Tortoli, Enrico.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 2, 2015, p. 510-515.

Research output: Contribution to journalArticle

Shahraki, AH, Çavuşoğlu, C, Borroni, E, Heidarieh, P, Koksalan, OK, Cabibbe, AM, Hashemzadeh, M, Mariottini, A, Mostafavi, E, Cittaro, D, Feizabadi, MM, Lazarevic, D, Yaghmaei, F, Molinari, GL, Camaggi, A & Tortoli, E 2015, 'Mycobacterium celeriflavum sp. Nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens', International Journal of Systematic and Evolutionary Microbiology, vol. 65, no. 2, pp. 510-515. https://doi.org/10.1099/ijs.0.064832-0
Shahraki, Abdolrazagh Hashemi ; Çavuşoğlu, Cengiz ; Borroni, Emanuele ; Heidarieh, Parvin ; Koksalan, Orhan Kaya ; Cabibbe, Andrea Maurizio ; Hashemzadeh, Mohamad ; Mariottini, Alessandro ; Mostafavi, Ehsan ; Cittaro, Davide ; Feizabadi, Mohamad Mehdi ; Lazarevic, Dejan ; Yaghmaei, Farhad ; Molinari, Gian Lorenzo ; Camaggi, Anna ; Tortoli, Enrico. / Mycobacterium celeriflavum sp. Nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 2. pp. 510-515.
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abstract = "Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0{\%} 16S rRNA gene sequence similarity, and 91.5–96.5{\%} similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9{\%}. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207T (5DSM 46765T5JCM 18439T).",
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AU - Shahraki, Abdolrazagh Hashemi

AU - Çavuşoğlu, Cengiz

AU - Borroni, Emanuele

AU - Heidarieh, Parvin

AU - Koksalan, Orhan Kaya

AU - Cabibbe, Andrea Maurizio

AU - Hashemzadeh, Mohamad

AU - Mariottini, Alessandro

AU - Mostafavi, Ehsan

AU - Cittaro, Davide

AU - Feizabadi, Mohamad Mehdi

AU - Lazarevic, Dejan

AU - Yaghmaei, Farhad

AU - Molinari, Gian Lorenzo

AU - Camaggi, Anna

AU - Tortoli, Enrico

PY - 2015

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