Mycobacterium florentinum sp. nov., isolated from humans

Enrico Tortoli, Laura Rindi, Khye S. Goh, Marja L. Katila, Alessandro Mariottini, Romano Mattei, Gianna Mazzarelli, Sini Suomalainen, Pirjo Torkko, Nalin Rastogi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Eight mycobacterial strains isolated during an 11 year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. Phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were Mycobacterium triplex and Mycobacterium lentiflavum. However, the lipids of the cell wall of the test isolates differed from those of the latter species by TLC and presented unique profiles by both GC and HPLC. Genotypic analysis showed that they had unique 16S rRNA gene and internal transcribed spacer (ITS) sequences, and could be differentiated from all other mycobacterial strains by PCR restriction analysis of hsp65. The strains presented high resistance to antimycobacterial drugs. The name Mycobacterium florentinum sp. nov. is proposed for this taxon, with strain FI-93171T (=DSM 44852T=CIP 108409T) as the type strain.

Original languageEnglish
Pages (from-to)1101-1106
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume55
Issue number3
DOIs
Publication statusPublished - May 2005

Fingerprint

Mycobacterium
new species
drug
Mycobacterium triplex
Mycobacterium lentiflavum
lipid
Sputum
rRNA Genes
Cell Wall
Names
gene
Lymph Nodes
High Pressure Liquid Chromatography
Lipids
Polymerase Chain Reaction
Lung
respiratory tract diseases
internal transcribed spacers
lymph nodes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Tortoli, E., Rindi, L., Goh, K. S., Katila, M. L., Mariottini, A., Mattei, R., ... Rastogi, N. (2005). Mycobacterium florentinum sp. nov., isolated from humans. International Journal of Systematic and Evolutionary Microbiology, 55(3), 1101-1106. https://doi.org/10.1099/ijs.0.63485-0

Mycobacterium florentinum sp. nov., isolated from humans. / Tortoli, Enrico; Rindi, Laura; Goh, Khye S.; Katila, Marja L.; Mariottini, Alessandro; Mattei, Romano; Mazzarelli, Gianna; Suomalainen, Sini; Torkko, Pirjo; Rastogi, Nalin.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 55, No. 3, 05.2005, p. 1101-1106.

Research output: Contribution to journalArticle

Tortoli, E, Rindi, L, Goh, KS, Katila, ML, Mariottini, A, Mattei, R, Mazzarelli, G, Suomalainen, S, Torkko, P & Rastogi, N 2005, 'Mycobacterium florentinum sp. nov., isolated from humans', International Journal of Systematic and Evolutionary Microbiology, vol. 55, no. 3, pp. 1101-1106. https://doi.org/10.1099/ijs.0.63485-0
Tortoli, Enrico ; Rindi, Laura ; Goh, Khye S. ; Katila, Marja L. ; Mariottini, Alessandro ; Mattei, Romano ; Mazzarelli, Gianna ; Suomalainen, Sini ; Torkko, Pirjo ; Rastogi, Nalin. / Mycobacterium florentinum sp. nov., isolated from humans. In: International Journal of Systematic and Evolutionary Microbiology. 2005 ; Vol. 55, No. 3. pp. 1101-1106.
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