Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens

Jesus Pais Ramos, Carlos Eduardo Dias Campos, Paulo Cesar de Souza Caldas, Nicole Victor Ferreira, Mariza Villas Boas da Silva, Paulo Redner, Creusa Lima Campelo, Sheila Ferreira Vale, Elizabeth Clara Barroso, Reginalda Ferreira de Melo Medeiros, Fátima Cristina Onofre Fandinho Montes, Teca Calcagno Galvão, Enrico Tortoli

Research output: Contribution to journalArticle

Abstract

Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples of a patient from the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the hsp65 gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the hsp65 (encoding the hypervariable region of the 65 kDa heat-shock protein) and rpoB (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species Mycobacterium celatum and Mycobacterium kyorinense. The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name Mycobacterium fragae. The type strain is HF8705T (= Fiocruz-INCQS/CMRVS P4051T = DSM 45731T).

Original languageEnglish
Pages (from-to)2583-2587
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART7
DOIs
Publication statusPublished - Jul 1 2013

Fingerprint

Mycobacterium
Mycobacterium celatum
new species
gene
genes
DNA-directed RNA polymerase
heat shock proteins
heat shock
Heat-Shock Proteins
Sputum
rRNA Genes
ribosomal RNA
Genes
Names
Brazil
RNA
Polymerase Chain Reaction
protein
testing
sampling

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Ramos, J. P., Campos, C. E. D., Caldas, P. C. D. S., Ferreira, N. V., da Silva, M. V. B., Redner, P., ... Tortoli, E. (2013). Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens. International Journal of Systematic and Evolutionary Microbiology, 63(PART7), 2583-2587. https://doi.org/10.1099/ijs.0.046862-0

Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens. / Ramos, Jesus Pais; Campos, Carlos Eduardo Dias; Caldas, Paulo Cesar de Souza; Ferreira, Nicole Victor; da Silva, Mariza Villas Boas; Redner, Paulo; Campelo, Creusa Lima; Vale, Sheila Ferreira; Barroso, Elizabeth Clara; Medeiros, Reginalda Ferreira de Melo; Montes, Fátima Cristina Onofre Fandinho; Galvão, Teca Calcagno; Tortoli, Enrico.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART7, 01.07.2013, p. 2583-2587.

Research output: Contribution to journalArticle

Ramos, JP, Campos, CED, Caldas, PCDS, Ferreira, NV, da Silva, MVB, Redner, P, Campelo, CL, Vale, SF, Barroso, EC, Medeiros, RFDM, Montes, FCOF, Galvão, TC & Tortoli, E 2013, 'Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens', International Journal of Systematic and Evolutionary Microbiology, vol. 63, no. PART7, pp. 2583-2587. https://doi.org/10.1099/ijs.0.046862-0
Ramos, Jesus Pais ; Campos, Carlos Eduardo Dias ; Caldas, Paulo Cesar de Souza ; Ferreira, Nicole Victor ; da Silva, Mariza Villas Boas ; Redner, Paulo ; Campelo, Creusa Lima ; Vale, Sheila Ferreira ; Barroso, Elizabeth Clara ; Medeiros, Reginalda Ferreira de Melo ; Montes, Fátima Cristina Onofre Fandinho ; Galvão, Teca Calcagno ; Tortoli, Enrico. / Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. PART7. pp. 2583-2587.
@article{554544511ae44da79954c1d4dfe08afc,
title = "Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens",
abstract = "Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples of a patient from the north-eastern Cear{\'a} state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the hsp65 gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the hsp65 (encoding the hypervariable region of the 65 kDa heat-shock protein) and rpoB (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species Mycobacterium celatum and Mycobacterium kyorinense. The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name Mycobacterium fragae. The type strain is HF8705T (= Fiocruz-INCQS/CMRVS P4051T = DSM 45731T).",
author = "Ramos, {Jesus Pais} and Campos, {Carlos Eduardo Dias} and Caldas, {Paulo Cesar de Souza} and Ferreira, {Nicole Victor} and {da Silva}, {Mariza Villas Boas} and Paulo Redner and Campelo, {Creusa Lima} and Vale, {Sheila Ferreira} and Barroso, {Elizabeth Clara} and Medeiros, {Reginalda Ferreira de Melo} and Montes, {F{\'a}tima Cristina Onofre Fandinho} and Galv{\~a}o, {Teca Calcagno} and Enrico Tortoli",
year = "2013",
month = "7",
day = "1",
doi = "10.1099/ijs.0.046862-0",
language = "English",
volume = "63",
pages = "2583--2587",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "PART7",

}

TY - JOUR

T1 - Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens

AU - Ramos, Jesus Pais

AU - Campos, Carlos Eduardo Dias

AU - Caldas, Paulo Cesar de Souza

AU - Ferreira, Nicole Victor

AU - da Silva, Mariza Villas Boas

AU - Redner, Paulo

AU - Campelo, Creusa Lima

AU - Vale, Sheila Ferreira

AU - Barroso, Elizabeth Clara

AU - Medeiros, Reginalda Ferreira de Melo

AU - Montes, Fátima Cristina Onofre Fandinho

AU - Galvão, Teca Calcagno

AU - Tortoli, Enrico

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples of a patient from the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the hsp65 gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the hsp65 (encoding the hypervariable region of the 65 kDa heat-shock protein) and rpoB (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species Mycobacterium celatum and Mycobacterium kyorinense. The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name Mycobacterium fragae. The type strain is HF8705T (= Fiocruz-INCQS/CMRVS P4051T = DSM 45731T).

AB - Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples of a patient from the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the hsp65 gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the hsp65 (encoding the hypervariable region of the 65 kDa heat-shock protein) and rpoB (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species Mycobacterium celatum and Mycobacterium kyorinense. The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name Mycobacterium fragae. The type strain is HF8705T (= Fiocruz-INCQS/CMRVS P4051T = DSM 45731T).

UR - http://www.scopus.com/inward/record.url?scp=84879802721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879802721&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.046862-0

DO - 10.1099/ijs.0.046862-0

M3 - Article

C2 - 23264503

AN - SCOPUS:84879802721

VL - 63

SP - 2583

EP - 2587

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - PART7

ER -