Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens

Jesus Pais Ramos, Carlos Eduardo Dias Campos, Paulo Cesar de Souza Caldas, Nicole Victor Ferreira, Mariza Villas Boas da Silva, Paulo Redner, Creusa Lima Campelo, Sheila Ferreira Vale, Elizabeth Clara Barroso, Reginalda Ferreira de Melo Medeiros, Fátima Cristina Onofre Fandinho Montes, Teca Calcagno Galvão, Enrico Tortoli

Research output: Contribution to journalArticlepeer-review

Abstract

Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples of a patient from the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the hsp65 gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the hsp65 (encoding the hypervariable region of the 65 kDa heat-shock protein) and rpoB (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species Mycobacterium celatum and Mycobacterium kyorinense. The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name Mycobacterium fragae. The type strain is HF8705T (= Fiocruz-INCQS/CMRVS P4051T = DSM 45731T).

Original languageEnglish
Pages (from-to)2583-2587
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART7
DOIs
Publication statusPublished - Jul 1 2013

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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