Mycobacterium insubricum sp. nov

Enrico Tortoli, Serena Baruzzo, Yvonne Heijdra, Hans Peter Klenk, Stefania Lauria, Alessandro Mariottini, Jakko van Ingen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Five independent strains, isolated from clinical samples but probably not responsible for disease, revealed phenotypic and genotypic features that appeared to exclude their belonging to any of the recognized Mycobacterium species. The strains, which are non-pigmented rapid growers, presented a cell-wall lipid pattern resembling those of Mycobacterium brumae and Mycobacterium fallax. Sequencing of the 16S rRNA, hsp65 and rpoB genes and the 16S-23S rRNA gene internal transcribed spacer (ITS) revealed that the strains are clearly distinct from every other Mycobacterium species. While the 16S rRNA and rpoB genes were characterized by a single sequevar, two sequevars were detected in hsp65 and three in the ITS. The divergence shown in the latter region was striking, in which only two short regions (less than 150 bp in all) were comparable with other mycobacteria, apart from Mycobacterium monacense and Mycobacterium gilvum. The PCR restriction analysis pattern of the novel strains also differs from any reported to date. The name Mycobacterium insubricum sp. nov. is proposed for the novel species; the type strain is FI-06250T (=DSM 45132T =CIP 109609T).

Original languageEnglish
Pages (from-to)1518-1523
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number6
DOIs
Publication statusPublished - 2009

Fingerprint

Mycobacterium
gene
new species
ribosomal RNA
Mycobacterium brumae
Mycobacterium fallax
Mycobacterium monacense
Mycobacterium gilvum
internal transcribed spacers
rRNA Genes
lipid
divergence
genes
Nontuberculous Mycobacteria
growers
cell walls
Cell Wall
Names
lipids
Lipids

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Tortoli, E., Baruzzo, S., Heijdra, Y., Klenk, H. P., Lauria, S., Mariottini, A., & van Ingen, J. (2009). Mycobacterium insubricum sp. nov. International Journal of Systematic and Evolutionary Microbiology, 59(6), 1518-1523. https://doi.org/10.1099/ijs.0.003459-0

Mycobacterium insubricum sp. nov. / Tortoli, Enrico; Baruzzo, Serena; Heijdra, Yvonne; Klenk, Hans Peter; Lauria, Stefania; Mariottini, Alessandro; van Ingen, Jakko.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 59, No. 6, 2009, p. 1518-1523.

Research output: Contribution to journalArticle

Tortoli, E, Baruzzo, S, Heijdra, Y, Klenk, HP, Lauria, S, Mariottini, A & van Ingen, J 2009, 'Mycobacterium insubricum sp. nov', International Journal of Systematic and Evolutionary Microbiology, vol. 59, no. 6, pp. 1518-1523. https://doi.org/10.1099/ijs.0.003459-0
Tortoli E, Baruzzo S, Heijdra Y, Klenk HP, Lauria S, Mariottini A et al. Mycobacterium insubricum sp. nov. International Journal of Systematic and Evolutionary Microbiology. 2009;59(6):1518-1523. https://doi.org/10.1099/ijs.0.003459-0
Tortoli, Enrico ; Baruzzo, Serena ; Heijdra, Yvonne ; Klenk, Hans Peter ; Lauria, Stefania ; Mariottini, Alessandro ; van Ingen, Jakko. / Mycobacterium insubricum sp. nov. In: International Journal of Systematic and Evolutionary Microbiology. 2009 ; Vol. 59, No. 6. pp. 1518-1523.
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