Mycobacterium saopaulense sp. Nov., a rapidly growing mycobacterium closely related to members of the mycobacterium chelonae– mycobacterium abscessus group

Christiane Lourenço Nogueira, Christopher M. Whipps, Cristianne Kayoko Matsumoto, Erica Chimara, Sara Droz, Enrico Tortoli, Denise De Freitas, Margo Cnockaert, Juan Carlos Palomino, Anandi Martin, Peter Vandamme, Sylvia Cardoso Leão

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Abstract

Five isolates of non-pigmented, rapidly growing mycobacteria were isolated from three patients and, in an earlier study, from zebrafish. Phenotypic and molecular tests confirmed that these isolates belong to the Mycobacterium chelonae–Mycobacterium abscessus group, but they could not be confidently assigned to any known species of this group. Phenotypic analysis and biochemical tests were not helpful for distinguishing these isolates from other members of the M. chelonae–M. abscessus group. The isolates presented higher drug resistance in comparison with other members of the group, showing susceptibility only to clarithromycin. The five isolates showed a unique PCR restriction analysis pattern of the hsp65 gene, 100 % similarity in 16S rRNA gene and hsp65 sequences and 1–2 nt differences in rpoB and internal transcribed spacer (ITS) sequences. Phylogenetic analysis of a concatenated dataset including 16S rRNA gene, hsp65, and rpoB sequences from type strains of more closely related species placed the five isolates together, as a distinct lineage from previously described species, suggesting a sister relationship to a group consisting of M. chelonae, Mycobacterium salmoniphilum, Mycobacterium franklinii and Mycobacterium immunogenum. DNA–DNA hybridization values >70 % confirmed that the five isolates belong to the same species, while values T (5CCUG 66554T5LMG 28586T5INCQS 0733T).

Original languageEnglish
Pages (from-to)4403-4409
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

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ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Nogueira, C. L., Whipps, C. M., Matsumoto, C. K., Chimara, E., Droz, S., Tortoli, E., Freitas, D. D., Cnockaert, M., Palomino, J. C., Martin, A., Vandamme, P., & Leão, S. C. (2015). Mycobacterium saopaulense sp. Nov., a rapidly growing mycobacterium closely related to members of the mycobacterium chelonae– mycobacterium abscessus group. International Journal of Systematic and Evolutionary Microbiology, 65(12), 4403-4409. https://doi.org/10.1099/ijsem.0.000590