Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species

Jakko van Ingen, Enrico Tortoli, Rangaraj Selvarangan, Marie B. Coyle, John A. Crump, Anne B. Morrissey, P. N Richard Dekhuijzen, Martin J. Boeree, Dick van Soolingen

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Abstract

'Mycobacterium sherrisii' is an undescribed species that appears to be emerging, in particular, among HIV-positive patients originating from Africa. To describe 'M. sherrisii', to ensure that the species name is validly published and to define its phylogenetic position, we collected 11 of these strains reported in five previous studies, and subjected them to biochemical identification, cell-wall mycolic acid analysis and sequencing of multiple housekeeping genes. The bacteria formed smooth and generally non-chromogenic colonies after 2-3 weeks of subculture at 24-37 °C; photochromogenic and scotochromogenic pigmentation were exhibited by three and two strains, respectively. The strains were positive for the heat-stable catalase test, but negative in tests for hydrolysis of Tween 80, nitrate reduction, β-glucosidase and 3-day arylsulfatase. Mycolic acid patterns, obtained by HPLC, resembled a trimodal profile similar to those of type strains of Mycobacterium simiae, Mycobacterium lentiflavum, Mycobacterium triplex and Mycobacterium genavense. The 16S rRNA gene sequences of the 11 strains differed by 4 bp (99.7 % similarity) from that of the type strain of the closest related species, M. simiae ATCC 25275 T. Levels of internal transcribed spacer (ITS) and partial hsp65 and rpoB gene sequence similarity between the two taxa were 95.8 % (271/283 bp), 97.5 % (391/401 bp) and 95.2 % (700/735 bp), respectively. On the basis of these results, we propose the formal recognition of Mycobacterium sherrisii sp. nov. The type strain is 4773 T (= ATCC BAA-832 T = DSM 45441 T).

Original languageEnglish
Pages (from-to)1293-1298
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number6
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Mycobacterium
gene
new species
Mycobacterium simiae
Mycolic Acids
acid
human immunodeficiency virus
Mycobacterium triplex
hydrolysis
Mycobacterium lentiflavum
Mycobacterium genavense
Arylsulfatases
Glucosidases
nitrate
phylogenetics
bacterium
Polysorbates
Essential Genes
nucleotide sequences
Pigmentation

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

van Ingen, J., Tortoli, E., Selvarangan, R., Coyle, M. B., Crump, J. A., Morrissey, A. B., ... van Soolingen, D. (2011). Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species. International Journal of Systematic and Evolutionary Microbiology, 61(6), 1293-1298. https://doi.org/10.1099/ijs.0.024752-0

Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species. / van Ingen, Jakko; Tortoli, Enrico; Selvarangan, Rangaraj; Coyle, Marie B.; Crump, John A.; Morrissey, Anne B.; Dekhuijzen, P. N Richard; Boeree, Martin J.; van Soolingen, Dick.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 6, 06.2011, p. 1293-1298.

Research output: Contribution to journalArticle

van Ingen, J, Tortoli, E, Selvarangan, R, Coyle, MB, Crump, JA, Morrissey, AB, Dekhuijzen, PNR, Boeree, MJ & van Soolingen, D 2011, 'Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species', International Journal of Systematic and Evolutionary Microbiology, vol. 61, no. 6, pp. 1293-1298. https://doi.org/10.1099/ijs.0.024752-0
van Ingen, Jakko ; Tortoli, Enrico ; Selvarangan, Rangaraj ; Coyle, Marie B. ; Crump, John A. ; Morrissey, Anne B. ; Dekhuijzen, P. N Richard ; Boeree, Martin J. ; van Soolingen, Dick. / Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species. In: International Journal of Systematic and Evolutionary Microbiology. 2011 ; Vol. 61, No. 6. pp. 1293-1298.
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