Three scotochromogenic Mycobacterium xenopi-like organisms were isolated from stream waters in Finland. These strains grew at 36-50 °C but not at 30 °C. One of the three strains was fully compatible with the M. xenopi type strain according to GLC-MS, biochemical tests, and 16S rDNA and 16S-23S rDNA internal transcribed spacer (ITS) sequencing. Two of the strains closely resembled M. xenopi in lipid analyses and biochemical tests, but analysis by GLC-MS verified the presence of two new marker fatty acids (2,4,6,x- tetramethyl-eicosanoic acid and 2,4,6,x,x-pentamethyl-docosanoic acid). The 16S rDNA and ITS region sequences of these two strains differed from those of M. xenopi and other previously described mycobacterial sequences. Therefore, the strains are regarded as new species of slow-growing mycobacteria, for which the name Mycobacterium botniense sp. nov. is proposed. The chemical, physical and microbiological quality of the water reservoirs of M. xenopi and M. botniense are described. As far as is known, this is the first time that M. xenopi has been isolated from natural waters. The strains of M. botniense sp. nov. (E347(T) and E43) have been deposited in the ATCC as strains 700701(T) and 700702, respectively.
|Number of pages||7|
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Publication status||Published - Jan 2000|
- 16S rDNA sequence
- Fatty acids
- ITS sequence
- Mycobacterium botniense sp. nov.
- Mycobacterium xenopi
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Ecology, Evolution, Behavior and Systematics