Mycobacterium xenopi and related organisms isolated from stream waters in Finland and description of Mycobacterium botniense sp. nov.

Pirjo Torkko, Sini Suomalainen, Eila Iivanainen, Merja Suutari, Enrico Tortoli, Lars Paulin, Marja Leena Katila

Research output: Contribution to journalArticlepeer-review

Abstract

Three scotochromogenic Mycobacterium xenopi-like organisms were isolated from stream waters in Finland. These strains grew at 36-50 °C but not at 30 °C. One of the three strains was fully compatible with the M. xenopi type strain according to GLC-MS, biochemical tests, and 16S rDNA and 16S-23S rDNA internal transcribed spacer (ITS) sequencing. Two of the strains closely resembled M. xenopi in lipid analyses and biochemical tests, but analysis by GLC-MS verified the presence of two new marker fatty acids (2,4,6,x- tetramethyl-eicosanoic acid and 2,4,6,x,x-pentamethyl-docosanoic acid). The 16S rDNA and ITS region sequences of these two strains differed from those of M. xenopi and other previously described mycobacterial sequences. Therefore, the strains are regarded as new species of slow-growing mycobacteria, for which the name Mycobacterium botniense sp. nov. is proposed. The chemical, physical and microbiological quality of the water reservoirs of M. xenopi and M. botniense are described. As far as is known, this is the first time that M. xenopi has been isolated from natural waters. The strains of M. botniense sp. nov. (E347(T) and E43) have been deposited in the ATCC as strains 700701(T) and 700702, respectively.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume50
Issue number1
Publication statusPublished - Jan 2000

Keywords

  • 16S rDNA sequence
  • Fatty acids
  • ITS sequence
  • Mycobacterium botniense sp. nov.
  • Mycobacterium xenopi

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

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